| Popis: |
Maps exist in the broadest range of scale possible, so this session will serve as a broad map of genotyping, with an emphasis on the basics of (1) DNA sequencing and (2) fragment analysis. There will be three speakers in this session, who will present an overview of the technologies and selected applications that are commonly found in core laboratories that study genes and DNA. The DNA-sequencing section will include the preliminary steps of template type and preparation, concentration determination, primer considerations, and sequencing reaction chemistries. There will also be a discussion of instrumentation, emphasizing capillary electrophoresis as it applies to both DNA sequencing and fragment analysis. In addition, the many reasons why researchers sequence DNA will be addressed, with specific examples. Finally, future directions for DNA-sequencing instrumentation will be mentioned, such as genome sequencing and micro-instruments. |
