| Popis: |
In this study, a new amperometric biosensor for creatinine determination was developed. For this purpose, apolypyrrole-polyvinylsulfonate (PPy-PVS) film was prepared by electropolymerization of pyrrole in apolyvinylsulfonate medium on a platinum plate. Creatinase and sarcosine oxidase enzymes were immobilizedon polypyrrole-polyvinylsulfonate film by cross-linking with glutaraldehyde. Contact angle images, SEM, andAFM of enzyme and non-enzyme surfaces were taken to determine the morphological and chemical propertiesof the Pt/PPy-PVS electrode. The determination of creatinine was made based on the oxidation of hydrogenperoxide at 0.4 V formed as a result of the enzymatic reaction on the surface of the prepared biosensor. The linear working range of the biosensor obtained was found between 5.0×10-6 M and 1.0×10-4 M. Using this lineargraph, the Km (observed) and Imax (observed) values for the double enzyme electrode system werecalculatedas 5.0×10-3 mM and 0.34 µA/min, respectively. Optimum working pH and temperature weredetermined as 8.0and 60 °C, respectively. The reusability and shelf life of the biosensor were determined. The effects ofinterferences in biological environments on biosensor response were investigated. For this purpose, uric acid,ascorbic acid, paracetamol, glycine, urea, and formaldehyde was used. The results have shown that theprepared biosensor has the potential to be used for creatinine determination in biological fluids.Keywords: Biological Fluid, Biosensor, Creatinine, Polypyrrole, Polyvinylsulfonate, Sarcosine Oxidase |
