CHANGES IN LEVELS OF SOME INNATE IMMUNE RESPONSE PARAMETERS, BLOOD VITAMINS E AND A IN STALIONS, FOLLOWING CASTRATION

Autor: Goundasheva, D., Hubenov, H., Kostov, K., Karadjov, T.
Rok vydání: 2005
Předmět:
Popis: We examined in stallions the impact of castration on total differential count of leukocytes and the classical pathway of complement activation (CPCA), blood vitamins A and E levels. The stallions were castrated with an emasculator, while in standing position, using the open technique. The number of leukocytes was counted in the Burker chamber, and the leukogram on blood smear. The neutrophil/lymphocyte ratio (N/L) was counted; CPCA was shown with Mayer’s method adapted for horses. Vitamins E and A concentrations were determined spectrophotometrically. The castration led to an increase in neutrophil bands number 3 hours later and on days 3 and 7; monocytes band number increased also 3 hours post castration. The N/L ratio increased on the 24th hour. The CPCA was reduced on the 0 hour, and the amount of vitamin E - on the 24th hour. The applied castration caused temporary and quantitative changes in the examined parameters of innate defence by suppressing its humoral component – complement (0 hour) and caused an increase of the cell elements – neutrophilsband and monocytes and the N/L ratio. It led to E hypovitaminosis.
Databáze: OpenAIRE