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Final Thesis Curriculum in Veterinary Medicine Sole ulcers (SU) are one of the most common conditions causing a remarkable amount of pain, lameness and welfare problems for dairy cattle. Subsequently to local pain and tissue damage the activation of systemic inflammatory response called acute phase response (APR) can be seen in cattle with SU. Increased concentrations of acute phase proteins (APP) like serum amyloid A (SAA) and Haptoglobin (Hp) together with increased rectal temperature and interleukin-6 (IL-6) concentrations are markers of APR. These can be used to evaluate the impact of SU on the inflammatory response of a cow. Dairy cows with SU (n = 18) and healthy animals (n = 30) were sampled during 14 days after hoof trimming. Serum samples (n = 144) and rectal temperature measurements (n =144) were collected beginning from the year of 2016 till the end of 2018. Both procedures were performed with seven-day interval (d0, d7 and d14) for each cow admitted into the study. Concentrations of SAA (p = 0.003), Hp (p = 0.037) and rectal temperature (p = 0.034) were higher in SU cows compared to control cows on d0. SAA concentrations in SU cows remained higher also at d7 and d14 samples (p = 0.005 and p = 0.014, respectively). Concentrations of Hp and rectal temperatures of the SU group declined within the study period and there were no significant differences to controls after d0. SAA concentrations in SU group were close to being statistically significantly lower (p = 0.052) on d14 compared to d0, Hp concentrations were lower (p = 0.035) on d14 compared to d0 and rectal temperatures were lower (p = 0.010) on d7 compared to d0. Statistically significant differences were not found in IL-6 concentrations between study groups and during the study period in SU cows. These results suggest that SU will initiate systemic APR in dairy cattle and APP can be used for monitoring disease process in SU cows. Tallahaavand (sole ulcer; SU) on lüpsilehmadel sageli esinev sõrahaigus mis põhjustab valu, lonkamist ja vähendab lehmade heaolu. Lisaks paiksele valule ja koekahjustusele käivitub ka süsteemne põletikuvastus (acute phase response; APR). Akuutse järgu valkude (acute phase proteins; APP)nagu seerumi amüloid A (SAA) ja haptoglobiini (Hp) kontsentratsiooni tõus koos kehatemperatuuri ja interleukiin-6 (IL-6) kontsentratsiooni tõusuga on APR tunnused. Nende abil on võimalik hinnata SU mõju lehma põletikureaktsioonile. Peale sõravärkimist SU diagnoosiga lüpsilehmadelt (n = 18) ning tervetelt kontroll-lehmadelt (n = 30) koguti 14 päeva jooksul vereproove ja mõõdeti kehatemperatuur alates värkimisest seitsme päevase intervalliga (päevad 0, 7 ja 14) . Uuring viidi läbi alates 2016 kuni 2018 aasta lõpuni. SU lehmadel olid 0 päeval SAA (p = 0.003), Hp (p = 0.037) ja rektaaltemperatuur (p = 0.034) kõrgemad võrreldes kontrollgrupiga. SAA kontsentratsioon SU lehmade püsis kõrgemal ka päevadel 7 ja 14 (p = 0.005 ja p = 0.014). SU lehmade Hp kontsentratsioon ja rektaaltemperatuur kahanesid aja jooksul, ning erinevusi võrreldes kontrollgrupiga ei olnud enam pärast 0 päeva. SU lehmade Hp kontsentratsioon oli 14-al päeval madalam (p = 0.035) võrreldes 0 päevaga ning rektaaltemperatuur oli 7-al päeval madalam (p = 0.010) võrreldes 0 päevaga. SAA kontsentratsioon oli peaaegu statistiliselt oluliselt väiksemad (p = 0.052) päeval 14 võrreldes 0 päevaga. Seerumi IL-6 kontsentratsioonid ei erinenud SU ja kontroll-lehmade vahel ega ka mõlema rühma sees uuringu jooksul. Selle uuringu tulemused näitavad, et SU käivitab lehmadel süsteemse põletikuvastuse ning APP-sid saab kasutada haiguse kulu jälgimiseks. |
