Utilizzo di un metodo citofluorimetrico per la conta di Listeria monocytogenes nei challenge test

Autor: D'Onofrio, F., Schirone, M., Lolli, M. G., Sacchini, F., Krasteva, I., DI Febo, T., Pomilio, F., Centorame, P., Tittarelli, M., Luciani, M., Iannetti, L.
Jazyk: italština
Rok vydání: 2022
Předmět:
The following study aims to compare Listeria monocytogenes enumeration performed by ISO 11290-2 and flow cytometry. Experiments were carried out on two L. monocytogenes strains grown at 8 and 4°C. The results highlighted that flow cytometry technique did not count the total number of bacterial cells enumerated by the official technique. Nevertheless it was evinced a constant relation between values obtained with both techniques: starting from a value of 4 log CFU/ml
flow cytometry permitted to enumerate 90-95% of the total L. monocytogenes cells counted by ISO 11290-2. This percentage decreased to 85-90% for 5 log CFU/ml
65-70% for 6 log UFC/ml
The following study aims to compare Listeria monocytogenes enumeration performed by ISO 11290-2 and flow cytometry. Experiments were carried out on two L. monocytogenes strains grown at 8 and 4°C. The results highlighted that flow cytometry technique did not count the total number of bacterial cells enumerated by the official technique. Nevertheless it was evinced a constant relation between values obtained with both techniques: starting from a value of 4 log CFU/ml
flow cytometry permitted to enumerate 90-95% of the total L. monocytogenes cells counted by ISO 11290-2. This percentage decreased to 85-90% for 5 log CFU/ml
65-70% for 6 log UFC/ml
60-65% for 7 log CFU/ml
and 55-60% for 8 log CFU/ml. No significant differences of the enumeration were observed as function of strains and growth temperature
and 55-60% for 8 log CFU/ml. No significant differences of the enumeration were observed as function of strains and growth temperature
60-65% for 7 log CFU/ml
Databáze: OpenAIRE
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