| Autor: |
Kemp, S.J., Maillard, Jean-Charles, Teale, A.J. |
| Jazyk: |
angličtina |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Animal Genetics |
| Popis: |
A polymorphism was detected in the 3' untranslated region of the bovine gamma-s-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products nom genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele-specific amplification. The two allele-specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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