| Autor: |
Golsaz Shirazi, F., Mohammadi, H., Amiri, M.M., Singethan, K., Xia, Y., Bayat, A.A., Bahadori, M., Rabbani, H., Jeddi-Tehrani, M., Protzer, U., Shokri, F. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
J. Gastroenterol. Hepatol. 29, 1083-1091 (2014) |
| Popis: |
BACKGROUND AND AIM: Antibodies against the "a" determinant of hepatitis B surface antigen (HBsAg) are able to neutralize circulating HBV particles and to prevent HBV infection. It has been proposed, that a single amino acid exchange may allow the virus to escape the immune response. We used a set of monoclonal antibodies (MAbs) to investigate whether a single mutation may account for virus escape from humoral immunity. METHODS: Nine murine HBsAg specific MAbs were raised. Reactivity of all antibodies with 14 recombinant mutants of HBsAg was assessed by ELISA. HBV infection of HepaRG cells was used to evaluate viral neutralization capacity of MAbs in vitro. RESULTS: All MAbs were able to inhibit the establishment of HBV infection in a dose dependent fashion, but recognition of HBsAg variants varied. The MAbs were classified into 3 subgroups based on their pattern of reactivity to the HBsAg variants. Accordingly, three MAbs showed weak reactivity ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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