Functionalized deoxynucleotides and DNA primers for electrochemical diagnostics of disease predispostions

Autor:	Debela AM, Ortiz M, Beni V, Lesage D, Cole R, O'Sullivan CK, Thorimbert S, Hasenknopf B
Přispěvatelé:	Universitat Rovira i Virgili
Rok vydání:	2017
Předmět:	Biotecnología Engenharias ii Astronomia / física Ciências agrárias i Interdisciplinar Química Engenharias iv Farmacia Engineering (all) Engenharias i Materiais Ciências ambientais Engineering (Miscellaneous) General engineering Engenharias iii
Zdroj:	Ecs Transactions Ecs Transactions. 77 (11): 1873-1883
DOI:	10.1149/07711.1873ecst
Popis:	Redox labeled DNAs are of increasing interest for the fabrication of next generation molecular tools. In the present work we are investigating the use of various redox labeled dNTPs, ddNTPs and DNA primers for use in detection of diseases. We have reported the use of Polyoxometalate (POM) labeled DNA primers and dNTPs for use in PCR and subsequently used for direct electrochemical detection of PCR products. The use of POM labeled DNAs in PCR enabled us to check the compatibility with polymerases and PCR incorporability of the modified DNAs. Furthermore we have investigated the solid-phase array based primer extension (é-PEX) with redox labelled ddNTPs (ferrocene (Fc), anthraquinone (AQ) phenothiazine (PTZ) and methylene blue (MB)) to prove the strategy of detection of single nucleotide polymorphisms using the labeled ddNTPs. This strategy will allow the development of cost-effective, rapid and user-friendly platform for the screening of known and unknown genetic mutations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______3351::34662f91da29d803727d2f170469a9a2 http://hdl.handle.net/20.500.11797/imarina3823741 Zobrazit plný text záznamu