| Autor: |
Quintana, Juan F., Sinton, Matthew C., Chandrasegaran, Praveena, Dubey , Lalit Kumar, Ogunsola, John, Samman, Moumen Al, Haley, Michael J, McConnell, Gail, Swar, Nono-Raymond Kuispond, Ngoyi, Dieudonne Mumba, Mabbott, Neil, MacLeod, Annette |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Quintana, J F, Sinton, M C, Chandrasegaran, P, Dubey, L K, Ogunsola, J, Samman, M A, Haley, M J, McConnell, G, Swar, N-R K, Ngoyi, D M, Mabbott, N & MacLeod, A 2023 ' The murine meninges acquire lymphoid tissue properties and harbour autoreactive B cells during chronic Trypanosoma brucei infection : Functional characterisation of the murine meninges during chronic infections ' bioRxiv . https://doi.org/10.1101/2023.04.30.538710 |
| Popis: |
The meningeal space is an important structure in the brain borders, which provides immunosurveillance for the central nervous system, but the impact of infections on the meningeal immune landscape is far from being fully understood. The extracellular protozoan parasite Trypanosoma brucei, which causes Human African Trypanosomiasis (HAT) or sleeping sickness, accumulate in the meningeal spaces, ultimately inducing severe meningitis and resulting in death if left untreated. Thus, sleeping sickness represents an attractive model to study immunological dynamics in the meninges during infection. Here, combining single cell transcriptomics and mass cytometry by time of flight (CyTOF), coupled with in vivo interventions, we found that chronic T. brucei infection triggers the development of ectopic lymphoid aggregates (ELAs) in the murine meninges during chronic infection. These infection-induced ectopic structures are defined by the presence of ER-TR7+ fibroblastic reticular cells (FRCs) and follicular dendritic cells (FDCs) that initiate a signalling cascade driving local T cell activation towards a T follicular helper (TFH)-like phenotype, as well as B cellclass switching. Furthermore, the GC-like B cells found in the infected meningesproduce high-affinity autoantibodies able to recognise mouse brain antigens. We found that systemic lymphotoxin β (LTβ) signalling blockade led to a significant depletion of meningeal FDC-like cells and autoreactive B cells, indicating that LTβ signalling is critical to induce and maintain local responses in the meninges. In humans, we identified the presence of autoreactive IgG antibodies able to recognise human brain lysates in the cerebrospinal fluid of second stage HAT patients compared to first stage HAT patients, consistent with our findings in experimental infections. Taken together, our data provide evidence that the meningeal immune response results in the acquisition of lymphoid tissue-like properties during chronic T. brucei infection, broadening our understanding of meningeal immunity in the context of chronic infections. These findings have wider implications for understanding the mechanisms underlying the formation ELAs during chronic inflammation resulting in autoimmunity in mice and humans, as observed in other autoimmune neurodegenerative disorders such as neuropsychiatric lupus and multiple sclerosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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