Brazilian Journal of Microbiology
| Autor: | Oliveira, I. C., Almeida, Rogeria Comastri de Castro, Hofer, Ernesto, Almeida, Paulo Fernando de |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFBA Universidade Federal da Bahia (UFBA) instacron:UFBA |
| Popis: | p. 1128-1136 Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-01-21T19:11:05Z No. of bitstreams: 1 Bacteriophage amplification assay ......pdf: 198889 bytes, checksum: 7596aa502f17606c1a7b8dc52cd0a421 (MD5) Made available in DSpace on 2013-01-21T19:11:05Z (GMT). No. of bitstreams: 1 Bacteriophage amplification assay ......pdf: 198889 bytes, checksum: 7596aa502f17606c1a7b8dc52cd0a421 (MD5) Previous issue date: 2012 A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes. Salvador |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|