Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Autor:	Bona,Claudine Maria de, Stelly,David, Miller Jr.,J. Creighton, Louzada,Eliezer Silva
Jazyk:	angličtina
Rok vydání:	2009
Předmět:	gamma rays Citrus Swinglea glutinosa asymmetric somatic hybridization fungi radiation hybrid mapping food and beverages hybrid cell lines
Zdroj:	Pesquisa Agropecuária Brasileira v.44 n.12 2009 Pesquisa Agropecuária Brasileira Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA
Popis:	The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=od______3056::b7d4b759aa7f4e1d979fd0ab9740c711 http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200008 Zobrazit plný text záznamu