Histological and histochemical description of the gastrointestinal tract of striped owl (Asio clamator) and of southern caracara (Caracara plancus) and immunohistochemistry for detection and identification of diffuse neuroendocrine system cell

Autor: Chaves, Rodrigo
Přispěvatelé: Pinheiro, Nadja Lima, Nascimento, Aparecida Alves do, Sales, Armando, Ferry, Fernando Raphael de Almeida, Mendes, Rosa Maria Marcos, Chagas, Walker Andr?
Jazyk: portugalština
Rok vydání: 2012
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2021-02-10T16:46:48Z No. of bitstreams: 1 2012 - Rodrigo Chaves.pdf: 7697680 bytes, checksum: cc5cd1ec62359abcecc75d58ede17b99 (MD5) Made available in DSpace on 2021-02-10T16:46:48Z (GMT). No. of bitstreams: 1 2012 - Rodrigo Chaves.pdf: 7697680 bytes, checksum: cc5cd1ec62359abcecc75d58ede17b99 (MD5) Previous issue date: 2012-07-02 For a System is established, it is essential that a larger number of species possess specific cells. For the chosen species no study is available in literature. There is no doubt that Diffuse Neuroendocrine System has an important role in the gastrointestinal function, however their phenotipic regulation in not completely elucidated, which indicates the importance of carrying out this search, which investigate species with different food habits. Whereas the basic premise of biological studies should be the Comparative Biology integrating different areas of knowledge, we sought to describe histologically the gastrointestinal tract wall of Asio clamator and Caracara plancus to evidenciate histochemically the presence of acids and neutral glycosaminoglycans and locate by immunohistochemistry Diffuse Neuroendocrine System cells with their respective peptides regulators. We hope demonstrate that this cells and their peptides are found in Falconiformes and Strigiformes species. To achieve these purposes, it was conducted an histological and histochemical (using Schiff periodic acid technique and alcian blue pH 2,5) study involving the description of gastrointestinal tract organs of two individuals from each of the following species Caracara plancus (Falconidae) e Asia clamator (Strigidae). Moreover, we detected and identified by immunohistochemistry cells which secrete gastrin, glucagon, somatostatin and serotonin. The cells were found in greater quantities in the proventriculus (stomach chemical) of the species investigated. The difference in habit (night and day) did not affect the distribution of cells of the Diffuse Neuroendocrine System. The results demonstrate that gastrointestinal tract wall organization is typical, as regards the mucosa, submucosa, muscle and serosa, since it presents adventitia in the esophagus and the ventricle without submucosal. The esophagus shows a stratified squamous epithelium without keratin containing few celular layers in e many of them in southern carcara. The mucosal glands of striped owl are tubular and can show ramifications. The mucosal glands in southern carcara are ?tubuloacinosas?. With respect to proper lamina, muscularis and adventitia, they are typical. The mucosal glands of esophagus were stained by histochemical techniques. Immunoreactive cells to sera used were not detected in esophagus. Proventriculus histological description is typical. The histochemical methods showed an mucosecretor epithelium. It was observed immunoreactive cells to gastrin, glucagon, serotonin and somatostatin in submucosal glands of striped owl. Immunoreactive cells to glucagon and somatostatin were found in southern carcara. In ventricle the histological description is typical. The histochemical showed a secretion product synthesized by tubular glands of mucosal region. It was not detected immunoreactive cells to anti-sera in any bird. The histological description of intestine is typical. The histochemical revealed the globet cells. In striped owl it was observed immunoreactive cells to glucagon and serotonin within epithelium and Lieberk?hn glands. Immunoreactive cells to glucagon was found in Lieberk?hn glands. Based on these results, it was found that different segments in the gastrointestinal tract of striped owl and of southern caracara have the same general structure of other vertebrates, and more specifically for avian; which cells producing glycosaminoglycans neutral and acidic are also observed in these species; and the location of endocrine cells in the gastrointestinal tract were essentially similar to those of other birds studied. O papel do Sistema Neuroend?crino Difuso na regula??o da fun??o gastrointestinal ? ineg?vel, por?m sua regula??o fenot?pica ainda n?o foi completamente esclarecida, demonstrando ent?o a import?ncia do estudo em esp?cies com diferentes h?bitos alimentares. Considerando que a premissa b?sica de estudos biol?gicos deve ser a Biologia Comparada, integrando diferentes ?reas de conhecimento, buscou-se: Descrever histologicamente a parede do trato gastrointestinal de Asio clamator e Caracara Plancus, evidenciar histoquimicamente a presen?a de glicosaminoglicanos ?cidos e neutros e localizar imunohistoquimicamente as c?lulas do Sistema Neuroend?crico Difuso com seus respectivos pept?deos reguladores, para demonstrar que estas c?lulas e seus pept?deos reguladores encontram-se tamb?m em esp?cies de Falconiformes e Strigiformes Para cumprir esses objetivos, foi realizado um estudo histol?gico (com a colora??o pela H-E) e histoqu?mico (com a t?cnica do ?cido peri?dico de Schiff e do alcian blue PH 2,5) para descri??o dos ?rg?os do trato gastrointestinal de 2 indiv?duos de cada uma das seguintes esp?cies, Caracara plancus (Falconidae) e Asia clamator (Strigidae), al?m do estudo imunohistoqu?mico para detec??o e identifica??o de c?lulas secretoras de gastrina, glucagon, serotonina e somatostatina. Os resultados demonstraram que a organiza??o da parede do trato gastrointestinal das esp?cies estudadas ? t?pica das aves, com mucosa, submucosa, muscular e serosa (advent?cia no es?fago e sem submucosa no ventr?culo). No es?fago, o epit?lio ? estratificado pavimentoso n?o queratinizado, apresentando poucas camadas celulares na coruja-orelhuda e muitas no carcar?. As gl?ndulas da mucosa tubulosas, apresentaram ramifica??es na coruja-orelhuda e mostraram-se tubuloacinosas no carcar?. A l?mina pr?pria, a muscular da mucosa, a submucosa, a camada muscular e a advent?cia s?o t?picas. As gl?ndulas da mucosa do es?fago foram marcadas pelas t?cnicas histoqu?micas. C?lulas imunorreativas aos anti-soros n?o foram detectadas no es?fago. A descri??o histol?gica do proventr?culo ? t?pica das aves. As t?cnicas histoqu?micas evidenciaram um epit?lio mucossecretor. Foram observadas c?lulas imunorreativas ? gastrina, ao glucagon, ? serotonina e ? somatostatina nas gl?ndulas da submucosa na coruja orelhuda e ao glucagon e ? somatostatina no carcar?. No ventr?culo a descri??o histol?gica ? t?pica. A histoqu?mica evidenciou um produto de secre??o sintetizado pelas gl?ndulas tubulares da regi?o mucosa. N?o foram detectadas c?lulas imunorreativas ao anti-soros em nenhuma das aves. A descri??o histol?gica dos intestinos ? t?pica. A histoqu?mica evidenciou as c?lulas caliciformes. Na coruja orelhuda, foram observadas no epit?lio e nas gl?ndulas de Lieberk?hn c?lulas imunorreativas ao glucagon e ? serotonina, e ? gastrina somente no epit?lio. No carcar?, foram observadas nas gl?ndulas de Lieberk?hn c?lulas imunorreativas ao glucagon. Com base nos resultados obtidos, concluiu?se que os diferentes segmentos do tubo digest?rio da coruja-orelhuda e do carcar? apresentam a mesma estrutura geral de outros vertebrados e mais especificamente de aves; que c?lulas produtoras de glicosaminoglicanos neutros e ?cidos tamb?m s?o observados nessas esp?cies; e que a localiza??o de c?lulas end?crinas no trato gastrointestinal foi essencialmente semelhante ? de outras aves estudadas.
Databáze: OpenAIRE
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