Transferability of heterologous microsatellite loci between species of Euterpe genus
| Autor: | AZÊVEDO, H. F., BENVINDO, F. D., CAVALCANTE, L. N., HAVERROTH, M., WADT, L. H. de O., CAMPOS, T. de |
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| Přispěvatelé: | Hellens Freires Azêvedo, Fundação Oswaldo Cruz, Universidade Federal do Acre (Ufac), MOACIR HAVERROTH, CPAF-Acre, LUCIA HELENA DE OLIVEIRA WADT, CPAF-Rondonia, TATIANA DE CAMPOS, CPAF-Acre. |
| Jazyk: | portugalština |
| Rok vydání: | 2017 |
| Předmět: |
Fitomejoramiento
Euterpe precatoria Ensayos de variedades Population dynamics Melhoramento genético vegetal Açaí Feijó (AC) Dinámica poblacional Variedade Loci Plant breeding Transferibilidade Acre Novo Segredo Microsatellite repeats Formoso Variación genética Genetic variation Marcador microssatélite Transferibilidad Variety trials Dinâmica populacional Amazonia Ocidental Transferability Repeticiones de microsatélite Variação Genética Western Amazon Marcador molecular |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| Popis: | Euterpe precatoria, popularly known as "açaí-do-amazonas", has agronomic, technological and economic potential and has been gaining prominence with the commercialization of its fruits. The production of this açaí-do-amazonas is based on extractivism and management practices have been recommended for sustainable collection. To recommend sustainable rates of fruit collection, it is essential to have information on the genetic variability of natural populations to monitor the recruitment and population dynamics. The objective of this study was to evaluate the transferability of microsatellite loci of E. edulis species to E. precatoria to confirm the applicability of these markers in genetic studies. Eighteen microsatellite loci developed for E. edulis were analyzed using 20 individuals from two natural populations (Formoso and Novo Segredo) of E. precatoria collected at Feijó, State of Acre, Brazil. All loci (100%) were amplified, and of these, nine (50%) were polymorphic. A total of 29 alleles were found in the Novo Segredo population, and 27 alleles were found in the Formoso population, ranging from two to five alleles per locus, with a mean of three alleles per locus. The expected heterozygosity in the Formoso population varied from 0.100 to 0.668, with an average of 0.421. In the Novo Segredo population, a variation between 0.100 and 0.710, with a mean of 0.418, was found. The observed heterozygosity values ranged from 0.100 to 0.800, with averages of 0.333 and 0.267 for Formoso and Novo Segredo, respectively. This set of markers will support further studies on the molecular characterization of the natural populations of E. precatoria and assist with the recommendation of sustainable management practices and strategies for the conservation and genetic improvement of this species. Made available in DSpace on 2017-11-08T23:30:27Z (GMT). No. of bitstreams: 1 26407.pdf: 489005 bytes, checksum: bd0871ea12532b6028d99cd4c2f45dc8 (MD5) Previous issue date: 2017-11-08 |
| Databáze: | OpenAIRE |
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