Antimicrobial effectiveness of grape seed extract against Enterococcus faecalis biofilm: confocal microscopy analysis

Autor: Fiallos, Nicole de Mello
Přispěvatelé: Sassone, Luciana Moura, Silva, Emmanuel João Nogueira Leal da, Hirata Júnior, Raphael, Fidel, Sandra Rivera, Prado, Maíra do
Jazyk: portugalština
Rok vydání: 2017
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UERJ
Universidade do Estado do Rio de Janeiro (UERJ)
instacron:UERJ
Popis: Submitted by Boris Flegr (boris@uerj.br) on 2021-01-07T15:06:39Z No. of bitstreams: 1 DISSERTACAO_FINAL_NICOLE_DE_MELLO_FIALLOS.pdf: 1357014 bytes, checksum: e166728fd72748c29241e8de36708d85 (MD5) Made available in DSpace on 2021-01-07T15:06:39Z (GMT). No. of bitstreams: 1 DISSERTACAO_FINAL_NICOLE_DE_MELLO_FIALLOS.pdf: 1357014 bytes, checksum: e166728fd72748c29241e8de36708d85 (MD5) Previous issue date: 2017-12-04 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior The limitations of chemical irrigants used in endodontics have encouraged further research on alternative substances to be used in endodontic treatment. Previous studies have suggested that grape seed extract (Vitis vinifera), an irrigant of natural origin, is effective against strains of Enterococcus faecalis. The aim of this study was to evaluate and quantify the degree of disinfection of dentin contaminated by E. faecalis biofilm after irrigation with Vitis vinifera grape seed extract 6,5% and to compare it with chlorhexidine (CHX) 2% and Sodium hypochlorite (NaOCl) 5,25% by confocal microscopy laser. Forty samples of dentin disks that will be contaminated with E. faecalis and incubated for a period of 21 days for biofilm formation were prepared. The samples were divided into different groups according to the irrigant tested after the period of contamination: NaOCl group, CHX group, Vitis vinifera group and control group . After irrigation, the dentin disks were stained with the fluorescent dye LIVE / DEAD BacLight and analyzed by laser confocal microscopy to determine the proportion of dead cells in the biofilm. The acquisition of the images and quantification of the biofilm was performed using the software LAS X. Normal distribution of the data was confirmed by Shapiro- Wilk test (p>0.05). Therefore, statistical analysis was performed using One-way analysis of variance. Post hoc pair-wise comparisons were performed using Tukey s test for multiple comparisons (P0,05). Por esse motivo, a análise estatística foi realizada utilizando-se One-way análise de variância. Comparações Post hoc pair-wise foram realizadas utilizando-se o teste Tukey para múltiplas comparações (P0,05). A irrigação com o extrato de semente de uva, vitis vinífera, apresentou atividade antimicrobiana sobre o biofilme de E. faecalis sendo esta inferior a apresenta pelo NaOCl e superior a apresentada pela CHX.
Databáze: OpenAIRE
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