Concentration of progesterone, metabolite of PGF2?, and embryomediate changes in endometrial gene expression in mares

Autor: Castro, Thadeu de
Přispěvatelé: Jacob, J?lio Cezar Ferraz, Mello, Marco Roberto Bourg de, Jesus, Vera Lucia Teixeira de, Pinna, Aline Emerim, Gomes, Gustavo Mendes
Jazyk: portugalština
Rok vydání: 2022
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-05-12T19:59:41Z No. of bitstreams: 1 2022 - Thadeu de Castro.pdf: 4366497 bytes, checksum: 4259d1d0362237461499fe38edef03c5 (MD5) Made available in DSpace on 2022-05-12T19:59:41Z (GMT). No. of bitstreams: 1 2022 - Thadeu de Castro.pdf: 4366497 bytes, checksum: 4259d1d0362237461499fe38edef03c5 (MD5) Previous issue date: 2022-02-23 CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior The aim of the present study was to characterize the relationship between progesterone (P4) and the metabolite of PGF2 (PGFM) during the interovulatory interval in nonbred mares, and and evaluate the gene expression in the endometrium on a day (Day 12, Day 0 = ovulation) of maximal embryo mobility. This study was carried out in two experiments and showed in two chapters consecutively. In the first experiment, were used nonbred mares (n = 8) and pregnant mares (n = 9) to evaluate the P4 and PGFM concentrations. Hypothesis 1 was that daily PGFM concentrations in nonbred mares increase at the intersection between the end of the rapid P4 increase and the gradual P4 decrease. Hypothesis 2 was that pregnant mares have low amplitude PGFM pulses during the days of the high amplitude pulses at luteolysis in nonbred mares. The first one daily blood samples, P4 increased between the day of ovulation (Day 0) and ~Day 6 and then gradually decreased until the beginning of luteolysis in the nonbred group. Before the beginning of luteolysis, there were no significant differences in P4 concentrations between the nonbred and early pregnancy. In the nonbred, PGFM concentration on the day before the beginning of luteolysis began to increase (P < 0.04) and reached a maximum mean (42.9 ? 11.6 pg/mL) on Day 14. In pregnancy, a novel increase in PGFM occurred from Day 12 to a maximum mean on Day 15 (16.7 ? 3.1 pg/mL). Daily PGFM concentrations were not different between the two groups until the increase just before luteolysis in the nonbred mares. During 8-h sessions of hourly blood sampling, the mean and maximum PGFM concentrations were significantly greater in nonbred than in pregnancy for each 8-h session on Days 13, 14, and 15. The pulses were similarly small for day-sets 4?7 and 9?11 in the nonbred and for all day-sets in pregnancy. In the second experiment, gene expression in the endometrium was compared among uterine horn with and without the embryo in pregnant mares (n = 13) and in the uterine horns of nonbred mares (n = 10). The hypothesis 3 was that the embryo locally upregulates PGE2 and PGF2? synthesis in the endometrium adjacent to the embryo. A cytobrush was used to collect an endometrial sample from the middle of each uterine horn. In nonbred mares, there was no difference for any of the considered gene expressions between the uterine horn ipsilateral and contralateral to the CL or for side (left vs right). For endometrial estrogen receptors, ESR1 was lower (P < 0.03) and ESR2 was greater (P < 0.04) for pregnant than nonbred mares. The mRNA abundance for PGE2 synthase (PGES) was greater (P < 0.05) in the horn with (1.40 ? 0.10) than without (0.89 ? 0.10) the embryo and was greater (P < 0.05) in the horn with the embryo than in the combined horns of nonbred mares (1.06 ? 0.10). It was conclued that (1) daily PGFM concentrations do not increase at the intersection between the end of the rapid P4 increase and the gradual P4 decrease; (2) pregnant mares have low amplitude PGFM pulses during the days of the high amplitude pulses at luteolysis in the nonbred mares;(3) the embryo locally upregulates PGE2 synthesis in the endometrium adjacent to the embryo. O objetivo do presente estudo foi caracterizar a rela??o entre as concentra??es de progesterona (P4) e metab?lito de PGF2? (PGFM) durante o per?odo interovulat?rio em ?guas n?o gestantes e comparar com o mesmo per?odo em ?guas gestantes, e avaliar efeitos local do embri?o na express?o g?nica endometrial durante o dia (dia 12, dia 0 = ovula??o) de m?xima mobilidade embrion?ria. Este estudo foi realizado em dois experimentos e ? apresentado em dois cap?tulos respectivamente. No primeiro experimento, foram utilizadas ?gua n?o gestantes (n = 8) e ?guas gestantes (n = 9) para avaliar as concentra??es de P4 e PGFM. A hip?tese 1 foi que as concentra??es di?rias de PGFM aumentam na interse??o entre o final do aumento de P4 e a diminui??o gradual de P4 (~ dia 6) em ?guas n?o gestantes. A hip?tese 2 foi que ?guas gestantes t?m pulsos de PGFM de baixa amplitude durante os dias correspondentes aos pulsos de alta amplitude na lute?lise em ?guas n?o gestantes. A concentra??o de P4 aumentou entre o dia da ovula??o e ~ dia 6, e em seguida, diminuiu gradualmente at? o in?cio da lute?lise em ?guas n?o gestantes. Antes do in?cio da lute?lise, n?o houve diferen?a significativa na concentra??o de P4 entre as ?guas n?o gestantes e gestantes. Nas ?guas n?o gestantes, a concentra??o de PGFM aumentou no dia anterior ao in?cio da lute?lise (P < 0,04) e atingiu m?dia m?xima (42,9 ? 11,6 pg/mL) no dia 14. Nas ?guas gestantes, um aumento in?dito de PGFM ocorreu a partir do dia 12 at? uma m?dia m?xima no dia 15 (16,7 ? 3,1 pg/mL). As concentra??es di?rias de PGFM n?o foram diferentes entre as ?guas n?o gestantes e gestantes at? antes da lute?lise nas ?guas n?o gestantes. Durante as sess?es de 8 horas de coleta das amostras, as concentra??es m?dia e m?xima de PGFM foram significativamente maiores nas ?guas n?o gestantes do que nas ?guas gestantes para cada sess?o de 8 horas nos dias 13, 14 e 15. Os pulsos foram igualmente pequenos para os conjuntos de dias 4?7 e 9?11 nas ?guas n?o gestantes e para todos os conjuntos de dias nas ?guas gestantes. No segundo experimento, a express?o g?nica do endom?trio foi comparada entre o corno uterino com e sem o embri?o de ?guas gestantes (n = 13) e nos cornos uterinos de ?guas n?o gestantes (n = 10). A hip?tese 3 foi que h? um aumento na produ??o de PGE2 e PGF2? no corno uterino adjacente ao embri?o. Escova de citologia uterina foi utilizada para coletar amostras no seguimento m?dio de cada corno uterino no dia 12. Nas ?guas n?o gestantes, n?o houve diferen?a na express?o g?nica de qualquer gene avaliado entre os cornos uterinos ipsilateral e contralateral ao CL. Para a express?o g?nica dos receptores de estr?geno, ESR1 foi menos (P < 0.03) e ESR2 foi mais (P < 0.04) expressado em ?guas gestantes do que em ?guas n?o gestantes. A express?o g?nica do gene relacionado a s?ntese de PGE2 (PTGES) foi maior (P < 0.05) no corno uterino com (1.40 ? 0.10) do que sem (0.89 ? 0.10) o embri?o e foi mais expressado (P < 0.05) no corno uterino com o embri?o do que nos cornos uterinos das ?guas n?o gestantes (1.06 ? 0.10). Conclui-se que (1) n?o h? um aumento de PGFM na interse??o entre o final do aumento de P4 e a diminui??o gradual de P4 (~ dia 6); (2) ?guas gestantes t?m pulsos de PGFM de baixa amplitude durante os dias correspondentes aos pulsos de alta amplitude na lute?lise em ?guas n?o gestantes; (3) o embri?o regula localmente a s?ntese de PGE2 no endom?trio adjacente ao embri?o.
Databáze: OpenAIRE
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