An??lise dos polimorfismos de base ??nica (SNP) em genes de IL-2 E IL-2R?? e o n??vel s??rico das citocinas do perfil TH1, TH2 E TH17 em pacientes infectados pelo v??rus da Hepatite C

Autor: Barthelemy, Jean Ludger
Přispěvatelé: Marie, Adriana Malheiro Alle, Monteiro, Andrea Tarrag??, Sadahiro, Aya, Mari??ba, Lu??s Andr?? Morais
Jazyk: portugalština
Rok vydání: 2020
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFAM
Universidade Federal do Amazonas (UFAM)
instacron:UFAM
ISSN: 4403-9670
Popis: Submitted by Jean Ludger Barthelemy (barthelemy.bibi@yahoo.fr) on 2021-03-21T16:07:24Z No. of bitstreams: 3 Disserta????o Jean Versao final d (1).pdf: 1609180 bytes, checksum: 09ca7d523db6741568ed722092be0421 (MD5) - Ata defesa Jean (1).pdf: 304886 bytes, checksum: da58cc4dfd3f472eec7d92feed85383d (MD5) carta de encaminhamento deposito-signed (1).pdf: 340034 bytes, checksum: 4d40ac467bc1b59b63e28bc4cc204325 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2021-03-21T23:48:58Z (GMT) No. of bitstreams: 3 Disserta????o Jean Versao final d (1).pdf: 1609180 bytes, checksum: 09ca7d523db6741568ed722092be0421 (MD5) - Ata defesa Jean (1).pdf: 304886 bytes, checksum: da58cc4dfd3f472eec7d92feed85383d (MD5) carta de encaminhamento deposito-signed (1).pdf: 340034 bytes, checksum: 4d40ac467bc1b59b63e28bc4cc204325 (MD5) Rejected by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br), reason: Na ficha catalogr??fica, corrigir o g??nero das palavras Orientador e Coorientador, para Orientadora e Coorientadora, uma vez que se referem a nomes femininos. on 2021-03-22T14:36:37Z (GMT) Submitted by Jean Ludger Barthelemy (barthelemy.bibi@yahoo.fr) on 2021-03-22T16:26:16Z No. of bitstreams: 3 - Ata defesa Jean (1).pdf: 304886 bytes, checksum: da58cc4dfd3f472eec7d92feed85383d (MD5) carta de encaminhamento deposito-signed (1).pdf: 340034 bytes, checksum: 4d40ac467bc1b59b63e28bc4cc204325 (MD5) Disserta????o Jean Ludger VF .pdf: 1609707 bytes, checksum: 3e101d44039670f208d91b6fab0c9579 (MD5) Approved for entry into archive by PPGBIOTEC Biotecnologia (ppgbiotecsecretaria@ufam.edu.br) on 2021-03-23T15:12:09Z (GMT) No. of bitstreams: 3 - Ata defesa Jean (1).pdf: 304886 bytes, checksum: da58cc4dfd3f472eec7d92feed85383d (MD5) carta de encaminhamento deposito-signed (1).pdf: 340034 bytes, checksum: 4d40ac467bc1b59b63e28bc4cc204325 (MD5) Disserta????o Jean Ludger VF .pdf: 1609707 bytes, checksum: 3e101d44039670f208d91b6fab0c9579 (MD5) Approved for entry into archive by Divis??o de Documenta????o/BC Biblioteca Central (ddbc@ufam.edu.br) on 2021-03-24T01:53:57Z (GMT) No. of bitstreams: 3 - Ata defesa Jean (1).pdf: 304886 bytes, checksum: da58cc4dfd3f472eec7d92feed85383d (MD5) carta de encaminhamento deposito-signed (1).pdf: 340034 bytes, checksum: 4d40ac467bc1b59b63e28bc4cc204325 (MD5) Disserta????o Jean Ludger VF .pdf: 1609707 bytes, checksum: 3e101d44039670f208d91b6fab0c9579 (MD5) Made available in DSpace on 2021-03-24T01:53:57Z (GMT). No. of bitstreams: 3 - Ata defesa Jean (1).pdf: 304886 bytes, checksum: da58cc4dfd3f472eec7d92feed85383d (MD5) carta de encaminhamento deposito-signed (1).pdf: 340034 bytes, checksum: 4d40ac467bc1b59b63e28bc4cc204325 (MD5) Disserta????o Jean Ludger VF .pdf: 1609707 bytes, checksum: 3e101d44039670f208d91b6fab0c9579 (MD5) Previous issue date: 2020-09-16 CNPQ - Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico Hepatitis C is a disease with a variable course where most cases result in chronic infection, which can lead to cirrhosis, hepatocarcinoma and liver transplantation. Only a minority of infected people are able to eliminate the virus during the acute phase without any therapeutic intervention. The factors explaining these differences are not yet well understood. In this sense, this study aimed to analyze the polymorphisms of IL-2 (rs2069762, rs4833248), and IL-2R?? (rs1003694), and the relationship these polymorphisms with the production of cytokines of the profile Th1, Th2 and Th17 in patients infected with the Hepatitis C virus. In this study, 149 samples from individuals infected with the hepatitis C virus and 100 from healthy individuals were analysed. DNA extraction was performed using the Brazol?? Kit. The determination of the allele frequencies of the polymorphisms was obtained by PCR-RFLP. The measurement of cytokines was performed by Flow Cytometry CBA (Cytometric Bead Array). Statistical analyzes were performed using the GraphPad Prism 5.0 program. The SNPs studied, were presented in Hardy-Weinberg equilibrium. No statistically significant were found in genotype and allele frequencies between the groups studied. The cytokines IL-2, IL-4, IL-6, IL-10, IL-17A, were increased in HCV + patients when compared to controls. The tumor necrosis factor was higher in the control group compared to the patient group. There was no statistically significant difference in the IFN-y level between the groups. Fibrosis> f2 was characterized by a high production of IL-2. The results obtained suggest the SNP IL2 rs4833248 and the SNP rs1003694 are not related to the development of Hepatitis C, the SNP IL-2 rs2069762 could be related to the chronicity of the disease and the high production of IL-2 in fibrosis > 2 is related to increased fibrogenic activity in patients with hepatitis C. A hepatite C ?? uma doen??a de curso vari??vel onde a maioria dos casos resulta em infec????o cr??nica, que pode levar a cirrose, hepatocarcinoma e transplante de f??gado. Somente uma minoria de pessoas infectadas ?? capaz de eliminar o v??rus durante a fase aguda sem qualquer interven????o terap??utica. Os fatores explicando essas diferen??as, ainda n??o s??o bem elucidados. Neste sentido, esse estudo teve como objetivos analisar os polimorfismos da IL-2 (rs2069762, rs4833248) e do IL-2R?? (rs1003694), e a rela????o desses polimorfismos com a produ????o das citocinas do perfil Th1, Th2 e Th17 em pacientes infectados pelo v??rus da Hepatite C. Foram analisados neste estudo, 149 amostras de indiv??duos infectados pelo v??rus da hepatite C e 100 de indiv??duos sadios. A extra????o de DNA foi feita, utilizando-se o Kit Brazol??. A determina????o das frequ??ncias al??licas dos polimorfismos foi obtida por PCR-RFLP. A dosagem das citocinas foi feita por Citometria de Fluxo CBA (Cytometric Bead Array). As an??lises estat??sticas foram realizadas com o programa GraphPad Prism 5.0. Os SNPs estudados, apresentaram-se em equil??brio de Hardy-Weinberg. N??o foram encontradas diferen??as estat??sticas significativas nas frequ??ncias genot??picas e al??licas entre os grupos estudados. As citocinas IL-2, IL-4, IL-6, IL-10, IL-17A, apresentaram-se aumentadas nos pacientes HCV+ quando comparado aos controles. O Fator de necrose tumoral foi maior no grupo controle em compara????o com o grupo de pacientes. N??o foi observada diferen??a estat??stica significativa no n??vel de IFN-y entre os grupos. A fibrose > f2 foi caracterizada por uma alta produ????o de IL-2. Os resultados obtidos sugerem que o SNP IL2 rs4833248 e o SNP rs1003694 n??o est??o relacionados ao desenvolvimento da Hepatite C, o SNP IL-2 rs2069762 poderia estar relacionado a cronicidade da doen??a e a alta produ????o de IL-2 na fibrose >2 est?? relacionada a uma atividade fibrog??nica aumentada em pacientes com hepatite C.
Databáze: OpenAIRE
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