The expression. of protein p53 in the morphogenesis of the gastroesophageal mucosa of Gallus gallus (Linnaeus, 1758)

Autor: Ventura, Adriana
Přispěvatelé: Pinheiro, Nadja Lima, Nascimento, Aparecida Alves, Chagas, Walker Andr?, Mendes, Rosa Maria Marcos
Jazyk: portugalština
Rok vydání: 2013
Předmět:
Zdroj: Biblioteca Digital de Teses e Dissertações da UFRRJ
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
instacron:UFRRJ
Popis: Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-04-09T15:33:16Z No. of bitstreams: 1 2013 - ADRIANA VENTURA.pdf: 2660504 bytes, checksum: a05168cb2a37d846567186bc2c60ab2c (MD5) Made available in DSpace on 2020-04-09T15:33:17Z (GMT). No. of bitstreams: 1 2013 - ADRIANA VENTURA.pdf: 2660504 bytes, checksum: a05168cb2a37d846567186bc2c60ab2c (MD5) Previous issue date: 2013-02-18 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. The ontogenesis of the gastroesophageal mucosa involves morphological changes that very according to the structure and function of each organ and events such as cell proliferation, differentiation and apoptosis. The aim of this study was to describe the histogenesis of the gastroesophageal mucosa of G. gallus, identify the alterations in the secretion pattern of glycosaminoglycans (GAGs), analyze the expression of protein p53 and correlate it with the morphological changes during development. Thirty-eight chicken embryos were used, classified as in stages 37 to 45 according to Hamburger and Hamilton (1951). The material collected was processed according to routine histological techniques and stained with hematoxylin-eosin for analysis of the tissue structure, with Gomori?s trichrome for identification of the conjunctive tissue and mainly collagen fibers, as well as with the histochemical techniques PAS and AB pH 2.5 for analysis of neutral and acid GAGs, respectively. For the immunohistochemical technique, 26 specimens were used, 24 embryos and the thymus of 2 adult animals. The epithelium covering the gastroesophageal mucosa was found to secrete mucus, while in the esophagus, the mucosa only secreted mucus in the initial stages of development. The esophageal and proventricular glands start to form in the initial stages of development (stage 37), while in the gizzard the tubular glands start to form only after the 15th day of incubation (stage 41). The differences in production of GAGs in these regions of the digestive tract are related to the advancement of morphogenesis in the developmental stages, the functions and physiological needs of each segment and the progressive adaptation of the organism to life after hatching. Protein p53 was expressed at crucial moments of development, in the esophagus (during remodeling of the cells to form the esophageal glands, stage 42 to 45), in the proventriculus (during differentiation of the mucosal epithelium, stage 39 to 45) and in the gizzard (during formation of the tubular glands, stage 42 to 45). The expression of p53 in the gastroesophageal mucosa occurs mainly in the stages when tissue remodeling starts and accompanies the differentiation of these cells until formation of glandular structures with characteristics similar to those in the adult bird. The differences in the production of GAGs in these regions of the gastroesophageal tract are related to development stages, functions, and physiological requirements of each segment, and to the gradual adjustment of the body to the post-hatching life. A ontog?nese da mucosa gastroesof?gica envolve altera??es morfol?gicas de acordo com a sua estrutura e fun??o de cada ?rg?o, orquestrados por eventos como a prolifera??o, diferencia??o e apoptose. A proposta deste estudo foi descrever a morfog?nese da mucosa gastroesof?gica de G. gallus, identificar as altera??es no padr?o de secre??o de glicosaminoglicanas (GAGs), analisar a express?o da prote?na p53 e correlacion?-la ?s altera??es morfol?gicas durante o desenvolvimento. Foram utilizados 38 embri?es de galinha, classificados nos est?dios 37 a 45, segundo Hamburger e Hamilton (1951). O material coletado foi processado segundo rotina histol?gica e submetidos ?s t?cnicas de colora??o pela hematoxilina-eosina para an?lise da estrutura tecidual e tricr?mico de Gomori para identifica??o do tecido conjuntivo e sobre tudo as fibras col?genas; e ?s t?cnicas histoqu?micas do PAS e AB pH 2,5 para an?lise de GAGs neutros e ?cidos, respectivamente. Para t?cnica de imunohistoqu?mica foram utilizados 26 esp?cimes, sendo 24 embri?es e o timo de 2 animais adultos. O epit?lio de revestimento da mucosa gastroesof?gica foi identificado como mucossecretor, sendo no es?fago principalmente nos est?dios iniciais, decrescendo com o desenvolvimento. As gl?ndulas esof?gicas e proventriculares come?am a se formar nos est?dios iniciais do desenvolvimento (est?dio 37), quanto na moela ?s gl?ndulas tubulares iniciam a sua forma??o somente ap?s o 15? dia de incuba??o (est?dio 41). A prote?na p53 ? expressa em momentos cruciais do desenvolvimento, no es?fago (durante a remodela??o das c?lulas para formar as gl?ndulas esof?gicas, est?dio 42 at? 45), no proventr?culo (enquanto o epit?lio da mucosa se diferencia (est?dio 39 ao 45) e na moela (durante a forma??o das gl?ndulas tubulares, est?dio 42 ao 45). A express?o da p53 na mucosa gastroesof?gica ocorre principalmente nos est?dios que iniciam a remodela??o tecidual e acompanham a diferencia??o destas c?lulas at? a forma??o de estruturas glandulares com caracter?sticas semelhantes ?s observadas no animal adulto. As diferen?as na produ??o de GAGs nessas regi?es do trato digest?rio est?o relacionadas aos avan?os do desenvolvimento nos est?dios de desenvolvimento, ?s fun??es e necessidades fisiol?gicas de cada segmento e ? adapta??o progressiva do organismo ? vida p?s-eclos?o.
Databáze: OpenAIRE
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