Cytokine

Autor: Lyra, Isa Menezes, Moura Neto, José Pereira de, Reis, Mitermayer Galvão dos, Barbosa, Cynara Gomes, Gonçalves, Marilda de Souza, Cerqueira, Bruno Antonio Veloso, Couto, Fábio David, Cajado, C., Santos, Wendell Vilas Boas, Dorea, M. J.
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Repositório Institucional da UFBA
Universidade Federal da Bahia (UFBA)
instacron:UFBA
ISSN: 1043-4666
DOI: 10.1016/j.cyto.2011.07.002
Popis: texto completo: acesso restrito. p. 312–317. Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-23T17:08:28Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S1043466611002201-main.pdf: 165270 bytes, checksum: 0e51b46fddf119fd4edc9fc0857708a9 (MD5) Made available in DSpace on 2012-02-23T17:08:28Z (GMT). No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S1043466611002201-main.pdf: 165270 bytes, checksum: 0e51b46fddf119fd4edc9fc0857708a9 (MD5) Previous issue date: 2011 Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) ÿ308G>A and Interleukin 8 (IL-8) ÿ251A>T gene polymorphisms, medical history and classical biomarkers in children with steady-state SCA. In total, 210 SCA patients aged 2–21 years and 200 healthy controls were studied. Gene polymorphisms, betaS-globin haplotypes and a 3.7-kb deletion in alpha2-thalassemia (a2-thal3.7 kb) were investigated by PCR/RFLP analysis, and cytokine levels were determined by ELISA. Splenomegaly (p = .032) was more prevalent among children younger than 5 years of age. The A allele of the TNF-alpha ÿ308G>A gene polymorphism and the presence of a2-thal3.7 kb were associated with an increase risk of splenic sequestration events (p = .001; p = .046), while the T allele of the IL-8 ÿ251A>T gene polymor-phism was considered to be a protective factor for splenomegaly events (p = .032). Moreover, the A allele of the TNF-alpha ÿ308G>A gene polymorphism was associated with high TNF-alpha levels (p = .021), and the hemoglobin F and hemoglobin S haplotypes were correlated with serum levels of IL-8. The logistic regression analysis showed significant effects of the TNF-alpha and IL-8 gene polymorphisms, betaS-globin gene haplotypes and a2-thal3.7 kb on the occurrence of splenic sequestration events. Our study empha-sizes that the identification of new genetic and immunological biomarkers and their associations with classical markers is an important strategy to elucidate the underlying causes of different SCA phenotypes and their effects on patient outcome.
Databáze: OpenAIRE
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