American Journal of Tropical Medicine and Hygiene

Autor: Lemaire, Denise Carneiro, Santos, Isis F. Magalhães, Andrade Filho, Antônio de Souza, Queiroz, Aristides Cheto de, Carvalho, Otavio Augusto Moreno de, Carmo, Theomira Mauadie Azevedo, Siqueira, Isadora C., Andrade, Débora M., Rego, Michely F., Guedes, Ana Paula T., Reis, Mitermayer Galvão dos
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Zdroj: Repositório Institucional da UFBA
Universidade Federal da Bahia (UFBA)
instacron:UFBA
Popis: Texto completo: acesso restrito. p. 294-298 Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-01-13T14:37:09Z No. of bitstreams: 1 DENISE C. LEMAIRE.pdf: 139362 bytes, checksum: 58a12a56c9ec4a974752aea98f4bd8c4 (MD5) Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-11-14T13:11:02Z (GMT) No. of bitstreams: 1 DENISE C. LEMAIRE.pdf: 139362 bytes, checksum: 58a12a56c9ec4a974752aea98f4bd8c4 (MD5) Made available in DSpace on 2014-11-14T13:11:02Z (GMT). No. of bitstreams: 1 DENISE C. LEMAIRE.pdf: 139362 bytes, checksum: 58a12a56c9ec4a974752aea98f4bd8c4 (MD5) Previous issue date: 2003 Cerebrospinal fluid (CSF) and serum samples from patients suspected of having neuroschistosomiasis (NS) were evaluated by an enzyme-linked immunosorbent assay. Monoclonal antibodies of various immunoglobulin isotypes (IgM, IgA, IgE, total IgG, IgG1, IgG2, IgG3, and IgG4) were used to detect antibodies against Schistosoma mansoni soluble egg antigen (SEA) and soluble worm adult preparation (SWAP). Of the 83 CSF samples tested, 55% were reactive toSEA (26% were reactive only toSEA and 29% tobo th SEA and SWAP), 34% were reactive toSWAP (5% only to SWAP and 29% to both SEA and SWAP), and 40% were not reactive with any antigen. Cases that tested positive for SWAP in CSF and negative in serum were not found. Samples with high specific IgG antibody titers were selected for immunoglobulin isotype profiling. In the CSF samples, the antibodies against SEA and SWAP were mainly IgM, IgG1, and IgG4, although other immunoglobulins were also detected. Interestingly, nine patients had high levels of IgG1 only in the CSF. These results suggest that there is local synthesis of IgG1, and that this isotype could be an important immunologic marker in the diagnosis of NS.
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