Acute effect of resistance exercise on the vascular actions of insulin
| Autor: | Fontes, Milene Tavares |
|---|---|
| Přispěvatelé: | Santos, Márcio Roberto Viana dos |
| Jazyk: | portugalština |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Repositório Institucional da UFS Universidade Federal de Sergipe (UFS) instacron:UFS |
| Popis: | The aim of our study was to evaluate the effects of a resistance exercise session on the vascular action of insulin in superior mesenteric artery of rats. Wistar rats (250-300g) were divided into 3 groups: control (CT, n = 20), electrically stimulated (ES, n = 5) and resistance exercise (RE, n = 20). The exercise was conducted in the apparatus of the squat, where the animals were subjected to 15 sets of 10 repetitions with 3 minutes rest between sets. The intensity was set at 70% of maximum load established by repetition maximum test performed 48h before the exercise session. The animals were kept suspended in EE squat machine and received the same intensity of electrical stimuli applied to the exercised animals. Immediately after the single resistance exercise session, the animals were anesthetized and killed by exsanguination, the superior mesenteric artery was removed and sectioned into rings (1-2 mm) which were mounted on tanks for isolated organ. The endothelium-dependent relaxation was obtained from concentration-response curves for insulin on rings precontracted with phenylephrine. After that, concentration response curves were obtained for groups CT response and ER, in the absence and/or presence of the following inhibitors: L-NAME (NOS inhibitor), TEA (inhibitor nonselective channel for K+), LY294002 (PI3K inhibitor), BQ123 ( ETA receptor antagonist), and Ouabain (inhibitor of Na+/K+-ATPase), concentration-response curves to KCl in the absence and/or presence of ouabain. According to the data obtained, we found that there was no significant difference in the relaxation induced by insulin between groups EE and CT, however, the animals in the ER showed a significant increase in relaxation when compared to CT group (p |
| Databáze: | OpenAIRE |
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