Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
| Autor: | Rodríguez, M.V., Mendiondo, G.M., Maskin, L., Gudesblat, G.E., Iusem, N.D., Benech-Arnold, R.L. |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
polymerase chain reaction
ABI5 hormone Blotting Western embryo Germination signal transducing adaptor protein phytohormone Sorghum bicolor bicolor Genes Plant Western blotting abscisic acid plant gene Plant Growth Regulators Gene Expression Regulation Plant imbibition genetics vegetable protein Sorghum Adaptor Proteins Signal Transducing Plant Proteins Pre-harvest sprouting growth development and aging seed dormancy article plant seed gene expression regulation Sorghum bicolor harvesting Hormone signalling inhibition developmental stage intraspecific variation physiology Seeds gene expression protein |
| Zdroj: | Ann. Bot. 2009;104(5):975-985 Biblioteca Digital (UBA-FCEN) Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
| Popis: | Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed. Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mendiondo, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
| Databáze: | OpenAIRE |
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