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This study evaluated the cytotoxicity of novel copper-chitosan (Chitosan-Cu) coatings for medical catheters. Catheters are commonly used in medical procedures but often cause inflammation and blood clots. Antibiotic-resistant bioactive coatings are being developed to address this, including Chitosan-Cu coatings which have shown antimicrobial activity. However, their toxicity to surrounding cells was unclear. The authors tested four Chitosan-Cu coatings with different copper concentrations (0-10% by volume) for cytotoxicity using L929 mouse fibroblast cells. Cell viability assays at 24 h and 72 h showed no significant toxicity for coatings up to 10% copper, with viabilities of 89-119% versus control. Coatings with higher copper levels showed slightly higher viabilities. Electron microscopy confirmed the surface morphology of coatings, while X-ray spectroscopy showed the presence of copper ions. Up to 10% copper, Chitosan-Cu coatings appeared non-toxic. These results support the safe use of these novel coatings in catheters to prevent infections and clotting, while avoiding side effects from excess copper. Further in vivo testing is still needed to verify the effectiveness and safety of these coatings before clinical use. |
