| Autor: |
Paloviita, P. (Pauliina), Hydén-Granskog, C. (Christel), Yohannes, D. A. (Dawit A.), Paluoja, P. (Priit), Kere, J. (Juha), Tapanainen, J. S. (Juha S.), Krjutškov, K. (Kaarel), Tuuri, T. (Timo), Võsa, U. (Urmo), Vuoristo, S. (Sanna) |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Popis: |
Small noncoding RNAs (sRNAs) play important roles during the oocyte-to-embryo transition (OET), when the maternal phenotype is reprogrammed and the embryo genome is gradually activated. The transcriptional program driving early human development has been studied with the focus mainly on protein-coding RNAs, and expression dynamics of sRNAs remain largely unexplored. We profiled sRNAs in human oocytes and early embryos using an RNA-sequencing (RNA-seq) method suitable for low inputs of material. We show that OET in humans is temporally coupled with the transition from predominant expression of oocyte short piRNAs (os-piRNAs) in oocytes, to activation of microRNA (miRNA) expression in cleavage stage embryos. Additionally, 3′ mono- and oligoadenylation of miRNAs is markedly increased in zygotes. We hypothesize that this may modulate the function or stability of maternal miRNAs, some of which are retained throughout the first cell divisions in embryos. This study is the first of its kind elucidating the dynamics of sRNA expression and miRNA modification along a continuous trajectory of early human development and provides a valuable data set for in-depth interpretative analyses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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