Lipopolysaccharide structures of helicobacter pylori genomic strains 26695 and j99, mouse model h. pylori sydney strain, h. pylori p466 carrying sialyl lewis x, and h. pylori ua915 expressing lewis b

core oligosaccharide-->lipid A) from five Helicobacter pylori strains: H. pylori 26695 and J99, the complete genome sequences of which have been published, the established mouse model Sydney strain (SS1), and the symptomatic strains P466 and UA915. All chemical and serological experiments were performed on the intact LPSs. H. pylori 26695 and SS1 possessed either a low-M, semi-rough-form LPS carrying mostly a single Le(y) type-2 blood-group determinant in the O-chain region covalently attached to the core oligosaccharide or a high-M-r smooth-form LPS, as did strain J99, with an elongated partially fucosylated type-2 N-acetyllactosamine (polyLacNAc) O-chain polymer, terminated mainly by a Le(x) blood-group determinant, connected to the core oligosaccharide. In the midst of semi-rough-form LPS glycoforms, H. pylori 26695 and SS1 also expressed in the O-chain region a difucosylated antigen, alpha-L-Fucp(1-3)-alpha-L-Fucp(1-4)-beta-D-GlcpNAc, and the cancer-cell-related type-1 or type-2 linear B-blood-group antigen, alpha-D-Galp(1-3)-beta-D-Galp(1-3 or 4)-beta-D-GlcpNAc. The LPS of H. pylori strain P466 carried the cancer-associated type-2 sialyl Le(x) blood-group antigen, and the LPS from strain UA915 expressed a type-1 Le(b) blood-group unit. These findings should aid investigations that focus on identifying and characterizing genes responsible for LPS biosynthesis in genomic strains 26695 and J99, and in understanding the role of H. pylori LPS in animal model studies. The LPSs from the H. pylori strains studied to date were grouped into specific glycotype families. -->
Přístupová URL adresa: https://explore.openaire.eu/search/publication?articleId=od______1513::c2f84eca88a6b1e954adfea2c335c222
http://hdl.handle.net/10379/9571
Rights: OPEN
Přírůstkové číslo: edsair.od......1513..c2f84eca88a6b1e954adfea2c335c222
Autor: Monteiro, Mario A., Appelmelk, Ben J., Rasko, David A., Moran, Anthony P., Hynes, Sean O., MacLean, Leann L., Chan, Ken H., Michael, Frank St, Logan, Susan M., O'Rourke, Jani, Lee, Adrian, Taylor, Diane E., Perry, Malcolm B.
Rok vydání: 2000
Předmět:
Popis: This study describes the molecular makeup of the cell-wall lipopolysaccharides (LPSs) (O-chain polysaccharide-->core oligosaccharide-->lipid A) from five Helicobacter pylori strains: H. pylori 26695 and J99, the complete genome sequences of which have been published, the established mouse model Sydney strain (SS1), and the symptomatic strains P466 and UA915. All chemical and serological experiments were performed on the intact LPSs. H. pylori 26695 and SS1 possessed either a low-M, semi-rough-form LPS carrying mostly a single Le(y) type-2 blood-group determinant in the O-chain region covalently attached to the core oligosaccharide or a high-M-r smooth-form LPS, as did strain J99, with an elongated partially fucosylated type-2 N-acetyllactosamine (polyLacNAc) O-chain polymer, terminated mainly by a Le(x) blood-group determinant, connected to the core oligosaccharide. In the midst of semi-rough-form LPS glycoforms, H. pylori 26695 and SS1 also expressed in the O-chain region a difucosylated antigen, alpha-L-Fucp(1-3)-alpha-L-Fucp(1-4)-beta-D-GlcpNAc, and the cancer-cell-related type-1 or type-2 linear B-blood-group antigen, alpha-D-Galp(1-3)-beta-D-Galp(1-3 or 4)-beta-D-GlcpNAc. The LPS of H. pylori strain P466 carried the cancer-associated type-2 sialyl Le(x) blood-group antigen, and the LPS from strain UA915 expressed a type-1 Le(b) blood-group unit. These findings should aid investigations that focus on identifying and characterizing genes responsible for LPS biosynthesis in genomic strains 26695 and J99, and in understanding the role of H. pylori LPS in animal model studies. The LPSs from the H. pylori strains studied to date were grouped into specific glycotype families.
Databáze: OpenAIRE
Externí odkaz: