| Autor: |
Pereira, Tifany, Caldeira, Maria João, Rodrigues, A., Alves, Madalena, Gonçalves, Viviane, Pinto, S., Montanha, Maria José, Teixeira, Cristina, Rodrigues, Carina |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Popis: |
Prioritizing healthcare workers (HCWs) for vaccination against the coronavirus disease 2019 (COVID-19) allowed to study the vaccine effectiveness in this professional group. The quantification of antibodies levels after vaccination among HCWs could give insights about SARS-CoV-2 protection. The aim of this study is to investigate SARS-CoV-2 immune response based on quantitation of antibodies against the spike receptor binding domain (RBD) of the S protein (anti-SRBD) and nucleocapside (anti-N) after the first vaccination stage in HCWs from the Local Health Unit of the Northeast of Portugal. Methods: Serum samples from 427 vaccinated HCW were tested for anti-SARS-CoV-2 immunoglobulin with chemiluminescent microparticle SARS-CoV-2 2 IgG I (anti-N) and IgG II (anti-SRBD) immunoassays detected by using ARCHITECT i1000SR (Abbot) equipment. Blood samples were collected between November and December of 2021, before the administration of 3rd vaccine dose. The chemiluminescent reaction anti-N immunoassay was expressed as an index classified as positive for values higher than 0.6. Anti-SRBD was quantified between 21.0 and 40,000.0 arbitrary units per ml (AU/mL) and values ≥ 50 AU/ml were considered positive. We obtained data about age, gender), infection with SARS-CoV-2 (PCR+), smoking habits and number of days between complete vaccination and blood sample collection Median, interquartile interval IIQ) and frequencies were obtained. Multivariate regression linear model was conducted to assess the factors affecting the values of anti-SRBD (logarithmized variable). Results: From all 427 HCW with median age 45.7 years [IIQ: 37.8-55.0], 89 (20.8%) were males, 89 (20.8%) had PCR+, 422 (98.8%) and 46 (21%) tested positive for IgG anti-SRBD and anti-N, respectively. The median of anti- SRBD was 737.3 (IIQ: 376.2-2270.1). According to the multivariate regression analysis, anti-SRBD values decrease as increase age (p = 0.004) and number of days since vaccination (p =0.004). Also, be a smoker decreases but a PCR+ increases anti-SRBD values. Conclusions/Recommendations: The SARS-CoV-2 antibody measurements suggested that quantitative anti-SRBD responses fall over the time (from days of complete immunization) and are lower among older vaccinated HCW. Projetos de Investigação Científica e Desenvolvimento Tecnológico (IC&DT). (Programa Testar com Ciência e Solidariedade: COVID-19 - 02/SAICT/2020/072562). info:eu-repo/semantics/publishedVersion |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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