| Popis: |
The reinforcing properties of alcohol (ethanol) are associated with activation of the mesolimbic dopamine system and the concomitant increase in dopamine in the nucleus accumbens (nAc). Changes in this system are thought to be a predominant underlying factor in promoting excessive alcohol intake and alcohol use disorder. We have previously shown that a simultaneous increase in endogenous taurine is required in order for ethanol to increase nAc dopamine levels, and hypothesize that taurine, which acts as an osmoregulator, is released in order to re-equilibrate the osmotic pressure. The intake of taurine has escalated over the last decade due to consumption of taurine-containing energy drinks, but whether a long-term intake of taurine induces adaptations influencing ethanol-induced dopamine elevation is not clear. Thus, the overall aim of this thesis was to investigate correlations between taurine and dopamine during ethanol exposure, with special focus on the nAc. To this end, behavioral tests were combined with neurochemical measurements and gene expression analysis performed in rodents. Our data show that systemically administrated taurine enters the CNS, a process that is not influenced by sub-chronic taurine treatment. Even though acute exposure does not increase locomotion, repeated exposure leads to behavioral sensitization to the drug, and taurine combined with caffeine potentiates ethanol-induced locomotion, a phenomenon previously linked to the reinforcing properties of the drug. By means of in vivo microdialysis we show that rats consuming high levels of ethanol respond with a blunted taurine elevation in response to acute ethanol treatment, and exhibit a lower dopamine tone compared to rats consuming low amounts of ethanol. At the same time, repeated taurine exposure does not influence the dopamine elevating properties of ethanol. By combining microdialysis with pharmacological and chemogenetic manipulations, we found that ethanol-induced taurine release is not action potential dependent and may involve astrocytes and volume regulated anion channels (VRACs). In conclusion, we suggest that increased nAc taurine levels following ethanol exposure mainly derives from astrocytes and involves VRACs, supporting an osmoregulatory role of taurine. Even though ethanol-induced dopamine release is not influenced by sub-chronic taurine exposure, taurine could contribute to the increase in alcohol consumption seen in humans drinking alcohol mixed with energy drinks. |
