Proteomic response of J774A.1 macrophages to biomedical Grade CoCr: Effect of wear
| Autor: | Sánchez-López, L., Ropero de Torres, N., Chico, Belén, de los Ríos Benítez, V., Escudero Rincón, María Lorenza, García-Alonso, M. C., Lozano, R.M. |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España) |
| Rok vydání: | 2022 |
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| Popis: | Summary of the scientific communication presented in the 32nd Congress of the European Society for Biomaterials ESB2022, held during 4 - 8 September 2022 in Bordeaux, France Upon implantation of any biomaterial, a biomaterial-based foreign response (B-FBR) is initiated. Immune cells are recruited to the implant site, being macrophages one of the key innate immune cell regulators of the biomaterial-based FBR. Implanted metallic materials may trigger cellular stress and they may induce adaptive responses in macrophages such as alteration of biological processes, depending on the cytotoxicity potential of the metallic compounds [1]. Moreover, upon implantation, metallic joint prostheses are subjected to degradation due to wear and corrosion, releasing metallic debris (metallic micro/nanoparticles and ions) which causes the risk of morbidity of biological and immunological responses. CoCr particles can cause local toxicity such as adverse local tissue reactions (ALTR, which includes aseptic lymphocyte-dominated vasculitis-associated lesion, necrosis, osteolysis and implant loosening, pseudo-tumors) and systemic toxicity (including cardiomyopathy, polycythemia, hypothyroidism, and neurological disorders) [2]. In this study, CoCr surfaces were subjected to wear-corrosion to generate metallic debris, and the worn surfaces impacted the macrophage response. Since an exhaustive characterization of macrophages in response to biomaterials is required, advances in new omics technologies, which allow massive expression analyses, may reveal new insights in the altered biological processes upon macrophage-biomaterial interactions [3]. Macrophage responses were characterized by a high-throughput proteomic approach. With this proteomic tool the biological responses of macrophages to worn CoCr surfaces were analyzed, elucidating the effect of wear-corrosion process on J774A.1 macrophages. The differential proteomics study allowed us to determine that: wear-corrosion process on CoCr surface impacted the macrophage response by inducing an increase in protein expression of Heme-oxygenase 1 (Hmox1) a well-known antioxidant protein involved in redox and metal ion homeostasis, which has been used as an indicator of cytotoxicity and oxidative stress induced by metallic biomaterials; J774A.1 macrophages were characterized by an antioxidant phenotype due to upregulation of REDOX mechanisms, which were activated probably to act as an adaptive response to counteract cellular oxidative stress and ROS associated to the wear-corrosion process applied on CoCr surface. This work was supported through RTI2018-101506-B-C31 and RTI2018-101506-BC33; and through PRE2019-090122, from Ministerio de Ciencia, Innovación y Universidades. |
| Databáze: | OpenAIRE |
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