| Autor: |
Allouba, M, Aguib, Y, Walsh, R, Afify, A, Theotokis, P, Galal, A, Halawa, S, Shorbagy, S, Ibrahim, AM, Kassem, HS, Ellithy, A, Buchan, R, Hosny, M, Whiffin, N, Elguindy, A, Anwer, S, Cook, SA, Ware, JS, Barton, PJ, Yacoub, M |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
Hypertrophic Cardiomyopathy (HCM) is an inherited disease characterized by genetic and phenotypic heterogeneity. MYH7 represents one of the main sarcomere-encoding genes associated with HCM. Missense variants in this gene cause HCM through gain-of-function actions, whereby variants produce an abnormal activated protein which incorporates into the sarcomere as a "poison peptide". Here we report a frameshift variant in MYH7, c.5769delG, that is associated with HCM in an Egyptian cohort (3.3%) compared with ethnically-matched controls. This variant is absent from previously published large-scale Caucasian HCM cohorts. We further demonstrate strong evidence of co-segregation of c.5769delG with HCM in a large family (LOD score: 3.01). The predicted sequence of the variant MYH7 transcript shows that the frameshift results in a premature termination codon (PTC) downstream of the last exon-exon junction of the gene that is expected to escape nonsense-mediated decay (NMD). RNA sequencing of myocardial tissue obtained from a patient with the variant during surgical myectomy confirmed the expression of the variant MYH7 transcript. Our analysis reveals a new mechanism of pathogenicity in the understudied Egyptian population whereby distal PTC in MYH7 may lead to the expression of an abnormal protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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