Istraživanje polimorfizma gena za κ-kazein i beta-laktoglobulin u buše i holštajnfrizijske pasmine mliječnih krava u Srbiji
| Autor: | Milan Maletić, Nevenka Aleksić, Branislav Vejnović, Dragan Nikšić, Milan Kulić, Božidar Đukić, Dragan Ćirković |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka Volume 66 Issue 3 |
| ISSN: | 1846-4025 0026-704X |
| Popis: | The aim of this study was to determine the distribution of κ-casein (κ-CN) and β-lactoglobulin (β-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of κ-CN and β-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that κ-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for κ CN. As for β-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for β-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and β-lactoglobulin) than HF cows. Cilj istraživanja bio je utvrditi raspodjelu genotipova kapa-kazeina (κ-CN) i beta-laktoglobulina (β-Lg) u autohtonih (buša) i mliječnih (holstein, HF) pasmina goveda primjenom PCR-RFLP. Za amplifikaciju κ-CN i β-Lg fragmenata gena korištene su specifične oligonukleotidne početnice. Nakon digestije posebnim endonukleazama (Hinf I i Hae III) genotipovi su određeni za oba gena u 18 buša i 19 HF krava. Rezultati su pokazali da je κ-CN gen utvrđen genotipom AA u 31,58 % HF krava, AB u 52,63 % krava, dok je genotip BB utvrđen u samo 15,79 % krava. Od krava pasmine buša 44,44 % je imalo AA genotip i 55,56 % genotip AB za κ-CN. Što se tiče β-Lg gena u HF pasmine, AA genotip pronađen je u 26,31 % krava, AB u 63,16 % i BB u 10,53 % krava. U krava pasmine buša sljedeći genotipovi su utvrđeni za β-Lg gen: AA u 44,44 % i AB u 55,56 % krava, dok BB genotip nije utvrđen. Ovi rezultati pokazuju da je u krava pasmine buša veća prisutnost A alelne forme za oba ispitivana gena (za k-CN i β-laktoglobulin) nego kod HF krava. |
| Databáze: | OpenAIRE |
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