The effects of resveratrol on SIRT2, SIRT3 expression levels and oxidative DNA damage in fumonisin-induced hepatotoxicity in BALB/c mice

Autor: Gulmez, Canan, Yalcin, Rıza, Kart, Asım, Atakisi, Emine, Tumakovich, Moldaliev Z., Atakisi, Onur
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Veterinarski arhiv
Volume 92
Issue 1
ISSN: 1331-8055
0372-5480
Popis: Oxidative stress, which is characterized by disruption of the oxidant/antioxidant balance, causes pathological processes, including toxicities induced by certain mycotoxins. The present study was designed to investigate the effects of resveratrol on sirtuin deacetylases (SIRT2 and SIRT3), nitric oxide (NO), reduced glutathione (GSH) and malondialdehyde (MDA) in fumonisin B1-induced hepatotoxicity. Regarding the experimental design, forty BALB/c mice were divided into four groups corresponding to the control, resveratrol (10 mg/kg, i.p), fumonisin B1 (2.25 mg/ kg, i.p) and resveratrol + fumonisin B1 (10 mg/kg + 2.25 mg/kg) groups. At the end of the 14 day-treatment, expression levels of SIRT2 and SIRT3 protein in the serum and liver were revealed by western blotting and antioxidant/oxidant activity analysis. SIRT2 and SIRT3 expression levels in the liver were significantly decreased by fumonisin B1 in comparison to the control. However, resveratrol supplementation coupled with fumonisin B1 increased the expression levels of SIRT2 and SIRT3, in relation to the fumonisin B1 treatments alone, but did not exhibit significant differences from those of the control group. As substantial indicators of stress and damage, the 8-OH-2-deoxyguanosine, NO and MDA levels of the liver tissue were assayed, and were higher in the fumonisin B1-treated groups, in relation to the control. As expected, resveratrol treatment significantly reduced the levels of NO and MDA in comparison to the fumonisin B1 treatments alone. Also, resveratrol attenuated the liver 8-OH-2- deoxyguanosine levels in the resveratrol + fumonisin B1 group. In conclusion, the findings revealed that resveratrol might possess protective effects against fumonisin-induced hepatotoxicity through modulation of the expression of sirtuin proteins, and by protecting the cell from oxidative/nitrosative stress.
Oksidacijski stres, koji obilježava poremećaj ravnoteže oksidansa i antiksidansa, uzrokuje patološke procese, uključujući toksičnost potaknutu određenim mikotoksinima. U ovom je radu istražen učinak resveratrola na sirtuin-deacetilazu (SIRT2 i SIRT3), dušikov oksid (NO), sniženi glutation (GSH) i malondialdehid (MDA) kod hepatotoksičnosti izazvane fumonizinom B1. Istraživanje je postavljeno tako da je 40 BALB/c miševa podijeljeno u četiri skupine: kontrolnu, skupinu koja je dobivala resveratrol (10 mg/kg, ip.), skupinu koja je dobivala fumonizin B1 (2,25 mg/kg, ip) i skupinu koja je dobivala resveratrol i fumonizin B1 (10 mg/kg+2,25 mg/kg). Nakon 14 dana određena je razina ekspresije proteina SIRT2 i SIRT3 metodom western blotting te analiza aktivnosti antioksidansa i oksidansa u serumu i jetri. Razina ekspresije SIRT2 i SIRT3 u jetri bila znakovito smanjena u skupini s fumonizinom B1 u usporedbi s kontrolnom skupinom. U skupini s dodatkom resveratrola i fumonizina B1, međutim, povećana je razina ekspresije SIRT2 i SIRT3 u usporedbi sa skupinom koja je dobivala fumonizin B1, no bez znakovite razlike između tih skupina i kontrolne skupine. Analizirani su ključni pokazatelji stresa i oštećenja, razine OH-2-deoksigvanozin, NO i MDA u tkivu jetre, koje su bile veće u skupini s fumonizinom B1, u usporedbi s kontrolnom skupinom. Kao što se očekivalo, primjena resveratrola znakovito je smanjila razine NO i MDA u usporedbi sa skupinom kojoj je primijenjen samo fumonizin B1. Također, resveratrol je smanjio razinu 8-OH-2- deoksigvanozina u jetri u skupini kojoj su dani i resveratrol i fumonizin. Rezultati pokazuju da bi resveratrol mogao imati zaštitni učinak u slučaju hepatotoksičnosti uzrokovane fumonizinom putem modulacije ekspresije sirtuin proteina i zaštite stanice od oksidacijskog/nitrosativnog stresa.
Databáze: OpenAIRE
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