| Autor: |
Rodamporn, Somphop, Beeby, Steve, Harris, Nick, Brown, Andrew, Chad, John |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Popis: |
Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10?sec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
