| Autor: |
Abdallah, Mohamed Fathi, Kilicarslan, Bilge, Girgin, Gözde, Baydar, Terken |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Mycotoxin workshop, 38th, Abstract book |
| Popis: |
Aflatoxins are one of the most significant food contaminants produced mainly by Aspergillus flavus and Aspergillus parasiticus. Aflatoxin M1 (AMF1), the hydrolyzed form and the main metabolite of Aflatoxin B1, is secreted into milk of dairy animals which are consuming aflatoxins contaminated feedstuffs. Searching through the internet database, only general screening methods such as ELISA have been used for detection of the mycotoxin in that area of Upper Egypt. Therefore, the aim of the study was to evaluate the occurrence of AFM1 in raw milk samples marketed in Assiut city. A total of 20 raw milk samples were purchased from local shops in Assiut, Upper Egypt. Skimmed milk samples, obtained from raw milk by centrifugation, were passed through Afla M1 immunoaffinity column for clean-up. AFM1 separation and detection was performed by using HPLC- FLD system. The limits of detection (LOD) and quantitation (LOQ) for AFM1 were 0.008 μg/kg and 0.02 μg/kg, respectively. The results showed that AFM1 is detected in all examined milk samples (100%) and 14 samples (70%) were above the maximum permissible level in the European Union (0.05 μg/kg). The concentrations ranged from 0.02μg/kg to 0.19 μg/kg except only one sample was under limit of quantification. In conclusion, more attention and strict regulations are highly recommended in order to control dairy milk contamination with mycotoxins. This work is financially supported by Hacettepe University Scientific Research Projects Coordination Unit (#014 D06 301 002-620). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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