Autor: |
Kremer, WDJ, Noordhuizen-Stassen, EN, Grommers, FJ, Daemen, AJJM, Henricks, PAJ, Brand, A, Burvenich, Christian |
Jazyk: |
angličtina |
Rok vydání: |
1993 |
Předmět: |
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Zdroj: |
JOURNAL OF DAIRY SCIENCE |
ISSN: |
0022-0302 |
Popis: |
Experimental mastitis was induced by inoculating rear right quarters of 10 healthy cows with 10(3) cfu of Escherichia coli. The chemotactic responses of peripheral blood polymorphonuclear leukocytes at d -6, -5, -2, -1, and immediately prior to inoculation were measured. Chemiluminescence of polymorphonuclear leukocytes was measured immediately prior to inoculation. Severity of the experimental mastitis was assessed by bacterial growth in the inoculated quarters. Results of this study indicated that severity of the experimental mastitis may be predicted by the chemotactic response in vitro of polymorphonuclear leukocytes isolated from the peripheral blood at d 2, d 1, and immediately prior to inoculation. The number of circulating polymorphonuclear leukocytes immediately prior to inoculation also showed a negative relationship with the severity of mastitis. No relationship existed between preinfection chemiluminescence of polymorphonuclear leukocytes and the severity of the experimental mastitis. Preinfection chemotactic response of polymorphonuclear leukocytes and preinfection numbers of circulating polymorphonuclear leukocytes appeared to be valuable as predictors of severity of experimental E. coli mastitis in cows. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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