Enteropathogenic Escherichia coli Infection Inhibits Intestinal Ascorbic Acid Uptake via Dysregulation of Its Transporter Expression
| Autor: | Heskett, Christopher W, Teafatiller, Trevor, Hennessey, Carly, Gareau, Melanie G, Marchant, Jonathan S, Said, Hamid M, Subramanian, Veedamali S |
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| Rok vydání: | 2021 |
| Předmět: |
Messenger
Clinical Sciences Ascorbic Acid Mice Enteropathogenic Escherichia coli SVCT2 SVCT1 Genetics Animals Humans 2.1 Biological and endogenous factors Vitamin C Intestinal Mucosa Aetiology Sodium-Coupled Vitamin C Transporters Escherichia coli Infections EPEC microRNA Gastroenterology & Hepatology Escherichia coli Proteins Biological Transport GRHPR Foodborne Illness Emerging Infectious Diseases Infectious Diseases Gene Expression Regulation Mutation RNA Caco-2 Cells Digestive Diseases Infection Biotechnology |
| Zdroj: | Digestive diseases and sciences, vol 66, iss 7 |
| Popis: | BackgroundEnteropathogenic Escherichia coli (EPEC) infection causes prolonged, watery diarrhea leading to morbidity and mortality. Although EPEC infection impacts nutrient transporter function and expression in intestinal epithelial cells, the effects of EPEC infection on intestinal absorption of ascorbic acid (AA) have not yet been investigated.AimsTo investigate the effect of EPEC infection on intestinal AA uptake process and expression of both AA transporters.MethodsWe used two experimental models: human-derived intestinal epithelial Caco-2 cells and mice. 14C-AA uptake assay, Western blot, RT-qPCR, and promoter assay were performed.ResultsEPEC (WT) as well as ΔespF and ΔespG/G2 mutant-infected Caco-2 cells showed markedly inhibited AA uptake, while other mutants (ΔescN, ΔespA, ΔespB, and ΔespD) did not affect AA uptake. Infection also reduced protein and mRNA expression levels for both hSVCT1 and hSVCT2. EPEC-infected mice showed marked inhibitory effect on AA uptake and decreased protein and mRNA expression levels for both mSVCT1 and mSVCT2 in jejunum and colon. MicroRNA regulators of SVCT1 and SVCT2 (miR103a, miR141, and miR200a) were upregulated significantly upon EPEC infection in both Caco-2 and mouse jejunum and colon. In addition, expression of the accessory protein glyoxalate reductase/hydroxypyruvate reductase (GRHPR), which regulates SVCT1 function, was markedly decreased by EPEC infection in both models.ConclusionsThese findings suggest that EPEC infection causes inhibition in AA uptake through a multifactorial dysregulation of SVCT1 and SVCT2 expression in intestinal epithelial cells. |
| Databáze: | OpenAIRE |
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