High-throughput miRNA sequencing of the human placenta: expression throughout gestation
| Autor: | Gonzalez, Tania L, Eisman, Laura E, Joshi, Nikhil V, Flowers, Amy E, Wu, Di, Wang, Yizhou, Santiskulvong, Chintda, Tang, Jie, Buttle, Rae A, Sauro, Erica, Clark, Ekaterina L, DiPentino, Rosemarie, Jefferies, Caroline A, Chan, Jessica L, Lin, Yayu, Zhu, Yazhen, Afshar, Yalda, Tseng, Hsian-Rong, Taylor, Kent, Williams, John, Pisarska, Margareta D |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male human transcriptome placenta Clinical Sciences Gestational Age Reproductive health and childbirth stable miRNAs uncomplicated pregnancies Pregnancy gestational differences Genetics Humans miRNA Gene Expression Profiling Human Genome Pregnancy Outcome Computational Biology High-Throughput Nucleotide Sequencing miRNome chorionic villi MicroRNAs miRNA atlas Gene Expression Regulation Female Transcriptome Biomarkers Biotechnology |
| Zdroj: | Epigenomics, vol 13, iss 13 |
| Popis: | Aim: To understand miRNA changes across gestation in healthy human placentae. This is essential before miRNAs can be used as biomarkers or prognostic indicators during pregnancy. Materials & methods: Using next-generation sequencing, we characterize the normative human placenta miRNome in first (n =113) and third trimester (n =47). Results &conclusion: There are 801 miRNAs expressed in both first and third trimester, including 182 with similar expression across gestation (p ≥0.05, foldchange ≤2) and 180 significantly different (false discovery rate 2). Of placenta-specific miRNA clusters, chromosome 14 miRNA cluster decreases across gestation and chromosome 19miRNA cluster is overall highly expressed. Chromosome 13 clusters are upregulated in first trimester. This work provides a rich atlas of healthy pregnancies to direct functional studies investigating the epigenetic differences in first and third trimester placentae. |
| Databáze: | OpenAIRE |
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