| Autor: |
Zambon, A C, Zhang, L Z, Minovitsky, S, Kanter, J R, Prabhakar, S, Salomonis, N, Vranizan, K, Dubchak, I, Conklin, B R, Insel, P A, Insel, Paul |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Zambon, A C; Zhang, L Z; Minovitsky, S; Kanter, J R; Prabhakar, S; Salomonis, N; et al.(2005). Gene expression patterns define key transcriptional events in cell-cycle regulation by cAMP and protein kinase A. Proceedings of the National Academy of Sciences of the United States of America, 102(24), 8561-8566. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/26b4v3gn |
| Popis: |
Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector mechanism, protein kinase A (PKA), on gene expression is not known. Here we show that treatment of murine wild-type S49 lymphoma cells for 24 h with 8-(4-chlorophenylthio)-cAMP (8-CPT-cAMP), a PKA-selective cAMP analog, alters the expression of approximate to 4,500 of approximate to 13,600 unique genes. By contrast, gene expression was unaltered in Kin(-) S49 cells (that lack PKA) incubated with 8-CPT-cAMP. Changes in mRNA and protein expression of several cell-cycle regulators accompanied cAMP-induced G(1)-phase cell-cycle arrest of wild-type S49 cells. Within 2 h, 8-CPT-cAMP altered expression of 152 genes that contain evolutionarily conserved cAMP-response elements within 5 kb of transcriptional start sites, including the circadian clock gene Per1. Thus, cAMP through its activation of PKA produces extensive transcriptional regulation in eukaryotic cells. These transcriptional networks include a primary group of cAMP-response element-containing genes and secondary networks that include the circadian clock. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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