| Autor: |
Ban, N, Escobar, C, Garcia, R, Hasel, K, Day, J, Greenwood, A, Mcpherson, A |
| Jazyk: |
angličtina |
| Rok vydání: |
1994 |
| Zdroj: |
Ban, N; Escobar, C; Garcia, R; Hasel, K; Day, J; Greenwood, A; et al.(1994). Crystal structure of an idiotype-anti-idiotype Fab complex. Proceedings of the National Academy of Sciences of the United States of America, 91(5), 1604-1608. doi: 10.1073/pnas.91.5.1604. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2vs0w8jb |
| DOI: |
10.1073/pnas.91.5.1604. |
| Popis: |
Anti-idiotypic monoclonal antibody 409.5.3 is raised against an antibody that neutralizes feline infectious peritonitis virus. This antibody, used as an immunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The crystal structure of the complex between anti-idiotypic Fab 409.5.3 and idiotypic Fab fragment of virus-neutralizing antibody has been solved by molecular replacement using real-space Patterson search and filtering by Patterson correlation-coefficient refinement. The structure has been refined to an R value of 0.21 based on 21.310 unique reflections between 40.0 and 2.9 A. The three-dimensional structure reveals extensive, specific interactions that involve 118 van der Waals contacts and at least 9 probable hydrogen bonds. The two Fabs are rotated 61° with respect to each other around the approximate long axis of the complex and are within 26° of being aligned along their major axes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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