Study on health and Immunity index of vaccinated Litopenaeus vannamei against white spot virus disease

Autor: Ghaednia, Babak, Mirbakhsh, M., Zendehbouy, A.A., Keshtkar, I., Nazary, M.A., Sabohi, M., Rajabifar, S., Shafaee, K., Raeesali, Gh., Zarin, E., Gorjifar, R., Heidary, M., Afsharnasab, M., Kakoolaki, S., Motamedi, F., Gharibi, Gh.
Jazyk: perština
Rok vydání: 2015
Předmět:
Popis: In this study, the inhibitory effect of Radio-Vaccine against white spot disease on white shrimp (Litopenaeus vannamei) was studied. This vaccine was prepared by Nuclear Science and Technology Research Institute of Atomic Energy Organization of Iran. 14,000 L. vannamei postlarvae were used in treatments after adaptation. Eight treatments, four positive and two negative controls were considered in triplicate. Initially, three meals a day for a month in the hours of 8, 16 and 24 were feeding. Three times in day at 8, 16 and 24 clock were feeding in first mount. Waste food and fecal remaining in the tanks were removed. In the second and third months of treatment, feeding frequency increased to three and four times in day which is at 6, 18 and 24 were given concentrate feed and Artemia nauplii at 12 clocks. Vaccination was carried out by immersion. Vaccination was performed in two doses with an interval of 10 to 14 days. For biometric testing and evaluation of immune cells throughout the experiment, three samples were taken randomly from the tanks. To assess the health status of shrimp, indices of Total Hemocyte Count and Total Plasma Protein was determined. Phagocytic activity of hemocytes was evaluated to assess immune setuation of shrimps. The results of this study showed that the shrimp immune system, as in many reports, the effect of recombinant vaccines and passive safety in memory as "Alternative adaptive immune responses" after experimental infection of white spot virus (WSSV) in wild and farmed shrimp is confirmed, will respond to vaccination against white spot disease. Iranian Fisheries Science Research Institute Published
Databáze: OpenAIRE