| Autor: |
Lim, Kenneth, Groen, Arnoud, Molostvov, Guerman, Lu, Tzongshi, Lilley, Kathryn S, Snead, David, James, Sean, Wilkinson, Ian B, Ting, Stephen, Hsiao, Li-Li, Hiemstra, Thomas F, Zehnder, Daniel |
| Přispěvatelé: |
Lilley, Kathryn [0000-0003-0594-6543], Wilkinson, Ian [0000-0001-6598-9399], Hiemstra, Thomas [0000-0002-2115-8689], Apollo - University of Cambridge Repository |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Popis: |
CONTEXT: α-Klotho has emerged as a powerful regulator of the aging process. To date, the expression profile of α-Klotho in human tissues is unknown, and its existence in some human tissue types is subject to much controversy. OBJECTIVE: This is the first study to characterize systemwide tissue expression of transmembrane α-Klotho in humans. We have employed next-generation targeted proteomic analysis using parallel reaction monitoring in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. RESULTS: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive, and neuronal tissues, was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, whereas liver did not reveal a detectable signal. These results were next confirmed by Western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho-expressing tissues were subjected to parallel reaction monitoring mass spectrometry (data deposited at ProteomeXchange, PXD002775) identifying peptides specific for the full-length, transmembrane α-Klotho isoform. CONCLUSIONS: The data presented confirm α-Klotho expression in the kidney tubule and in the artery and provide evidence of α-Klotho expression across organ systems and cell types that has not previously been described in humans. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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