| Autor: |
Kunz, L.I., Lapperre, T.S., Snoeck-Stroband, J.B., Budulac, S.E., Timens, W., van Wijngaarden, S., Schrumpf, J.A., Rabe, K.F., Postma, D.S., Sterk, P.J., Hiemstra, P.S. |
| Přispěvatelé: |
Faculteit der Geneeskunde |
| Jazyk: |
angličtina |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Respiratory Research, 12(1). BioMed Central |
| ISSN: |
1465-9921 |
| Popis: |
BACKGROUND: Macrophages have been implicated in the pathogenesis of COPD. M1 and M2 macrophages constitute subpopulations displaying pro- and anti-inflammatory properties. We hypothesized that smoking cessation affects macrophage heterogeneity in the lung of patients with COPD. Our aim was to study macrophage heterogeneity using the M2-marker CD163 and selected pro- and anti-inflammatory mediators in bronchoalveolar lavage (BAL) fluid and induced sputum from current smokers and ex-smokers with COPD. METHODS: 114 COPD patients (72 current smokers; 42 ex-smokers, median smoking cessation 3.5 years) were studied cross-sectionally and underwent sputum induction (M/F 99/15, age 62+/-8 [mean+/-SD] years, 42 (31-55) [median (range)] packyears, postbronchodilator FEV1 63+/-9% predicted, no steroids past 6 months). BAL was collected from 71 patients. CD163+ macrophages were quantified in BAL and sputum cytospins. Pro- and anti-inflammatory mediators were measured in BAL and sputum supernatants. RESULTS: Ex-smokers with COPD had a higher percentage, but lower number of CD163+ macrophages in BAL than current smokers (83.5% and 68.0%, p=0.04; 5.6 and 20.1 x10*4/ml, p=0.001 respectively). The percentage CD163+ M2 macrophages was higher in BAL compared to sputum (74.0% and 30.3%, p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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