Peritoneal dialysis fluid bioincompatibility and new vessel formation promote leukocyte-endothelium interactions in a chronic rat model for peritoneal dialysis
| Autor: | Schilte, Margot N, Fabbrini, Paolo, Wee, Piet M Ter, Keuning, Eelco D, Zareie, Mohammad, Tangelder, Geert-Jan, Van Lambalgen, Anton A, Beelen, Robert H J, Van Den Born, Jacob |
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| Přispěvatelé: | Groningen Institute for Organ Transplantation (GIOT), Groningen Kidney Center (GKC) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Endothelium Vascular/drug effects Microscopy Video Peritoneal Dialysis/adverse effects Dialysis Solutions/adverse effects Peritoneum/blood supply Rats Microcirculation/drug effects Disease Models Animal Neutrophils/drug effects Peritonitis/etiology Animals Leukocytes/drug effects N-Formylmethionine Leucyl-Phenylalanine/pharmacology Rats Wistar Leukocyte Rolling/drug effects |
| Zdroj: | Microcirculation, 17(4), 271-280 |
| ISSN: | 1073-9688 |
| Popis: | Peritoneal dialysis (PD)-induced peritonitis leads to dysfunction of the peritoneal membrane. During peritonitis, neutrophils are recruited to the inflammation site by rolling along the endothelium, adhesion, and transmigration through vessel walls. In a rat PD-model, long-term effects of PD-fluids (PDF) on leukocyte-endothelium interactions and neutrophil migration were studied under baseline and inflammatory conditions. Rats received daily conventional-lactate-buffered PDF (Dianeal), bicarbonate/lactate-buffered PDF (Physioneal) or bicarbonate/lactate buffer (Buffer) during five weeks. Untreated rats served as control. Baseline leukocyte rolling and N-formylmethionyl-leucyl-phenylalanine (fMLP) induced levels of transmigration in the mesentery were evaluated and quantified by intra-vital videomicroscopy and immunohistochemistry. Baseline leukocyte rolling was unaffected by buffer treatment, approximately 2-fold increased after Physioneal and 4-7-fold after Dianeal treatment. After starting fMLP superfusion, transmigrated leukocytes appeared outside the venules firstly after Dianeal treatment (15 minutes), thereafter in Physioneal and Buffer groups (20-22 minutes), and finally in control rats (>25 minutes). Newly formed vessels and total number of transmigrated neutrophils were highest in Dianeal-treated animals, followed by Physioneal and Buffer, and lowest in control rats and correlated for all groups to baseline leukocyte rolling (r = 0.78, P < 0.003). This study indicates that the start of inflammatory neutrophil transmigration is related to PDF bio(in)compatibility, whereas over time neutrophil transmigration is determined by the degree of neo-angiogenesis. |
| Databáze: | OpenAIRE |
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