| Autor: |
Patruno, Teresa Paolucci, Vanessa Pino, Osama Elsallabi, Marialucia Gallorini, Gianantonio Pozzato, Alessandro Pozzato, Paola Lanuti, Victor Machado Reis, Mirko Pesce, Andrea Pantalone, Roberto Buda, Antonia |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Antioxidants; Volume 12; Issue 7; Pages: 1358 |
| ISSN: |
2076-3921 |
| DOI: |
10.3390/antiox12071358 |
| Popis: |
This study aimed to investigate the anti-inflammatory effects of Quantum Molecular Resonance (QMR) technology in an in vitro model of osteoarthritis-related inflammation. The study used THP-1-derived macrophages stimulated with lipopolysaccharide and hyaluronic acid fragments to induce the expression of inflammatory cytokines and nitrosative stress. QMR treatment inhibited COX-2 and iNOS protein expression and activity and reduced NF-κB activity. Furthermore, QMR treatment led to a significant reduction in peroxynitrite levels, reactive nitrogen species that can form during inflammatory conditions, and restored tyrosine nitration values to those similar to sham-exposed control cells. We also investigated the effect of QMR treatment on inflammasome activation and macrophage polarization in THP-1-derived macrophages. Results showed that QMR treatment significantly decreased NLRP3 and activated caspase-1 protein expression levels and downregulated IL-18 and IL-1β protein expression and secretion. Finally, our findings indicate that QMR treatment induces a switch in macrophage polarization from the M1 phenotype to the M2 phenotype. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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