アグロバクテリウム法によるタバコ巻葉ウイルス外被タンパク質遺伝子のタバコへの導入と導入遺伝子のホモ化に関する研究
| Autor: | Takashi, Nikami, Taniguchi Miho, Kadotani Naoto, Morisima Hiroko, Tanda Seinosuke, Ikegami Masato |
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| Jazyk: | japonština |
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| Popis: | タバコNicotiana tabacum cv Burley21へAgrobacterium tumefaciensを用いてジェミニウイルス科(Geminiviridae)に属するtobacco leaf curl virus(TbLCV)の外被タンパク質遺伝子を導入した。外被タンパク質遺伝子を導入したタバコを自殖して後代系統を養成し,T1世代,T2世代における分離比を算定することにより,導入遺伝子のコピー数を推定した。分離比から1コピー,2コピー,3コピーと推定される形質転換タバコ系統を得た。1コピー,2コピーと考えられる形質転換植物については更に自殖を繰り返し,導入遺伝子をホモに持つ組換え系統を得た。 Coat protein gene of Tobacco leaf curl virus was introduced to Nicotiana tabacum cv Burley21 by mediation with Agrobacterium tumefaciens. Transgenic plants obtained were inbreed, offspring plants were harvested and then segregation ratios were computed of T1 generation plants using resistance for hygromycin as a marker trait. For results obtained, copy numbers of introduced coat protein gene were inferred. We obtained transgenic plants containing one copy, two copies and three copies of introduced coat protein gene. The homozygous lines were obtained by inbreeding of transgenic tobacco plants which contained one or two copies of introduced coat protein gene. |
| Databáze: | OpenAIRE |
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