Biological effects of high LET heavy ions in human blood lymphocytes and mouse cells
| Autor: | Hande, Prakash, Zeegers, D., Venkatesan, S., Seah, M., Velmurugan, P., Koh, S., Sethu, S., Jayapal, M., Banerjee, B., Hirakawa, H., Liu, Cuihua, Okayasu, Ryuichi, Fujimori, Akira, Hirakawa, Hirokazu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Popis: | Human blood cells and mouse embryonic fibroblasts (MEFs) were exposed to Iron (Fe) ions (500 Mev/u, LET-200 keV, 0.1 to 2.0 Gy) at the National Institute of Radiological Sciences in Chiba, Japan. Chromosomal alterations were analysed using PNA-FISH with telomere/centromere specific probes as well as by multi-colour-FISH. Gene expression profiles were generated from blood lymphocytes to identify signature genes of exposure to Fe ions. A dose dependent (0.1 to 1.0 Gy) increase in the extent of DNA damage and in the percentage of chromosome aberrations was observed. Fe ions produced more complex chromosome aberrations as compared to -rays. Irradiation with low doses of Fe ions induced differential gene expression of 3378 and 4437 genes at 2- and 24-h post-irradiation respectively. MEFs with dysfunctional telomeres or DNA repair displayed a higher sensitivity to micronuclei induction and chromosomal damage to Fe ions (0 to 2 Gy) as compared to wild-type cells. Currently, we are studying the effects of Fe ions on genome stability in vivo in wild type and DNA repair deficient mice (supported by MEXT Grant-in-Aid for Scientific Research on Innovative Areas “Living in Space”). Collectively, it is observed that Fe ions induced varied molecular and cellular changes including chromosomal aberrations and differential gene expression, which are distinct from those of -radiation exposure. The results obtained here will, hopefully, provide us with the functional relevance of early biomarkers of exposure to space radiation as well as in the manifestation of heavy ion therapy. 第62回日本放射線影響学会大会 |
| Databáze: | OpenAIRE |
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