| Autor: |
Tsuzuki, Teruhisa, Isoda, Takuro, Piao, J, Yamauchi, Kazumi, Egashira, Akinori, Sakamoto, Katsumi, Kura, Shinobu, Nakabeppu, Yusaku, Nakatsu, Yoshimichi |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
9th European Biological Inorganic Chemistry Conference : EUROBIC 9 : 2-6 September, 2008 Wroclaw, Poland. :7-14 |
| Popis: |
Oxygen radicals are produced through normal cellular metabolism, and formation of such radicals is further enhanced by ionizing radiation and by various chemicals. Among various classes of oxidative DNA damage, 8-oxo-7,8-dihydroguanine (8-oxoG) is the most abundant, and appears to play important roles in mutagenesis and carcinogenesis. We have focused on following the two enzymes. MTH1 protein hydrolyzes 8-oxo-dGTP to monophosphate in the nucleotide pool, thereby preventing occurrence of transversion mutations. On the other hand, MUTYH protein, having adenine/2-hydroxyadenine DNA glycosylase activity, is expected to prevent G:C to T:A transversions, by excising adenine from G:A mismatches induced by 8-oxoG and 2-OH-A. To analyze the function of the mammalian MTH1 and MUTYH proteins in vivo, we established gene-knockout mice for these two enzymes, and investigated spontaneous tumorigenesis as well as mutagenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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