Coupling factor 6 の血管内皮細胞におけるケモカイン受容体発現に及ぼす影響
| Autor: | Suzuki, Akiko, Osanai, Tomohiro, Tanaka, Makoto, Endo, Tomohide, Murakami, Kazuo, Tomita, Hirofumi, Okumura, Ken |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | 弘前医学. 65(2-4):119-127 |
| ISSN: | 0439-1721 |
| Popis: | Objectives: Vascular endothelial cells are exposed to an acidic pH, but its influence on chemokine receptors expressed in the vascular endothelial cells is unclear. We investigated the role of coupling factor 6 (CF6), a novel stimulator of proton importer, in the regulation of chemokine receptors in the vascular endothelial cells. Methods and Results: In microarray analysis, there were the increased expression of CC chemokine receptor 9 (CCR9) and CX3C chemokine receptor 1 (CX3CR1) and the decreased expression of CXC chemokine receptor 4 (CXCR4) in the human umbilical vascular endothelial cells (HUVEC) that were exposed to CF6. The ratio of CXCR4 to GAPDH mRNA was decreased in HUVEC that were exposed to either CF6 at 10-7M or hypoxia to a similar degree. Apoptotic cells, measured by annexin-V propidium iodide kit, were increased in HUVEC that were exposed to CF6 for 24 hours in normoxia. Conclusions: CF6 influences the expression of chemokine receptors and induces apoptosis in the vascular endothelial cells. 弘前医学. 65(2-4), 2014, p.119-127 |
| Databáze: | OpenAIRE |
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