On-line rapid purification of [13N]N2 gas for visualization of nitrogen fixation and translocation in nodulated soybean

Autor: Yin, Yonggen, Satomi, Ishii, Nobuo, Suzui, Keisuke, Kurita, Yuta, Miyoshi, Naotsugu, Nagasawa, Mitsumasa, Taguchi, Naoki, Kawachi
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Applied Radiation and Isotopes. 151:7-12
ISSN: 0969-8043
Popis: Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [13N]N2 gas and obtained real-time images of N fixation in an intact soybean plant. The purification efficiency was ~81.6% after decay correction. Our method provides accurate signals of N fixation and allows free changes to the tracer gas composition to suit different experimental designs.
Databáze: OpenAIRE