| Autor: |
Matsuo, Yoichiro, Izumi, Yoshinobu, Sakamoto, Ayako, Hase, Yoshihiro, Sato, Katsuya, Shimizu, Kikuo |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Zdroj: |
Quantum Beam Science. 3(3):14 |
| ISSN: |
2412-382X |
| Popis: |
Ion beams have several significantly interesting features, such as high biological effect and unique mutation spectrum. In this study, the effects of double strand breaks and 8-oxo-2'-deoxyguanosine (8-oxodG) caused by ion-beam irradiation were examined. We irradiated repair-gene-inactive strains rad52, ogg1, and msh2 using carbon ions beams, analyzed the lethality and characterized the mutations. The present observations suggested that nucleotide incorporation of oxidative damage gave only a limited effect on cell viability and genome fidelity. We also detected a hotspot at around +79 to +98 in URA3 in wild-type and mutant lines, suggesting the presence of a mutation-susceptible region. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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