FISH analysis of chromosomal aberrations in splenocytes of Trp53-heterozygous mice exposed to both chronic restraint-induced stress and Fe-particle irradiation
| Autor: | Vares, Guillaume, LIU, Qiang, Katsube, Takanori, Wang, Bing, Tanaka, Kaoru, Ninomiya, Yasuharu, Hirakawa, Hirokazu, Liu, Cuihua, Maruyama, Kouichi, Nakajima, Tetsuo, Fujimori, Akira, Nenoi, Mitsuru |
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| Jazyk: | japonština |
| Rok vydání: | 2019 |
| Popis: | Astronauts develop psychological stress (PS) during space flight because of enclosed environment, zero gravity and altered light-dark cycles. At the same time, they are exposed to cosmic rays including high atomic number and energy (HZE) particles such as iron-56 (Fe) ions. A pioneering study showed that chronic restraint-induced PS (CRIPS) could attenuate Trp53 functions and increase carcinogenesis induced by low linear energy transfer (LET) gamma-rays in Trp53-heterozygous (Trp53+/-) mice. To elucidate possible modification effects from CRIPS on high LET HZE particles-induced health consequences, Trp53+/- C57BL/6N male mice (six-week-old) were restrained 6 hours per day for consecutive 28 days, and total-body irradiation (TBI) of Fe ions at a dose of 0.1 or 2 Gy was given on the 8th day. Chromosomal aberrations (CAs) in splenocytes were observed by fluorescence in situ hybridization (FISH) at the end of the 28-day restraint regimen. Induction of PS in our experimental model was confirmed by an increase in urinary corticosterone level. CRIPS reduced splenocyte number per spleen but Fe TBI. Fe TBI alone increased CAs significantly at 2.0 Gy but 0.1 Gy. Exposure to CRIPS alone didn’t induce CAs but concurrent exposure to CRIPS and Fe TBI showed a tendency of increase in the frequency of CAs as compared to exposure to Fe TBI alone. Further analyses are required to confirm the modification effects from CRIPS on Fe TBI-induced CAs. This work was partially supported by the MEXT Grant-in-Aid for 日本放射線影響学会第62回大会 |
| Databáze: | OpenAIRE |
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