Creating peroxidase-oxidase fusion enzymes as toolbox for cascade reactions

Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: ChemBioChem. 18:2226-2230
ISSN: 1439-7633
1439-4227
Popis: In this work we prepared a set of bifunctional oxidase-peroxidases by fusing four distinct oxidases to a peroxidase. While such fusion enzymes have not been observed in nature, they could be expressed and purified with good yields. Characterization revealed that the artificial enzymes retained the capability to bind the two required cofactors and were catalytically active as oxidase and peroxidase. The peroxidase fusions of alditol oxidase and chitooligosaccharide oxidase could be used for selective detection of xylitol and cellobiose with a detection limit in the low µM range. The peroxidase fusions of eugenol oxidase and 5-hydroxymethylfurfural oxidase could be used for dioxygen-driven one-pot two-step cascade reactions to convert vanillyl alcohol into divanillin and eugenol into lignin oligomers, respectively. The designed oxidase-peroxidase fusions represent attractive biocatalysts that allow efficient biocatalytic cascade oxidations that only require molecular oxygen as oxidant.
Databáze: OpenAIRE
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